Interplay among recombination, replication, segregation, and chromatin structure.

重组、复制、分离和染色质结构之间的相互作用。

• 批准号: 17080011
• 负责人: OHTA Kunihiro
• 金额: $ 41.41万
• 依托单位: The University of Tokyo (2007-2009)The Institute of Physical and Chemical Research (2005-2006)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17080011/
• 关键词: 組換え; 減数分裂; 複製; クロマチン; ヒストン; 姉妹染色体; 染色体; エビジェネティクス; 細胞周期; ヒストン修飾; 酵母; 相同組換え; DNA複製; 姉妹染色体結合; セントロメア; Spo11

Meiotic recombination plays crucial roles in establishment of genetic diversity and gametes formation. The regulation of meiotic recombination involves various DNA-related processes. This project uncovered that meiotic recombination initiator Spo11 is activated by phosphorylation of a Spo11-accesspry protein Mer2 by Cdc7-Dbf4 kinase, which is a master regulator of DNA replication cycle. We also discovered that Spo11 distribution is regulated by meiotic chromatid cohesion protein Rec8 and replication. In addition, we found several factors involved in chromatin modification required for the activation of meiotic recombination hotspots. Finally, we discovered a new class of long mRNA-type non-coding RNA involved in chromatin control for the stress-induced gene activation in fission yeast.

减数分裂重组在遗传多样性的建立和配子的形成中起着至关重要的作用。减数分裂重组的调控涉及各种DNA相关过程。该项目发现，减数分裂重组启动子Spo 11通过Cdc 7-Dbf 4激酶磷酸化Spo 11-accesspry蛋白Mer 2而激活，Cdc 7-Dbf 4激酶是DNA复制周期的主调节因子。我们还发现Spo 11的分布受减数分裂染色单体凝聚蛋白Rec 8和复制的调节。此外，我们还发现了几个参与减数分裂重组热点激活所需的染色质修饰的因素。最后，我们发现了一类新的长mRNA型非编码RNA参与染色质控制的压力诱导的基因激活裂殖酵母。

项目成果

Actin-related protein Arp6 influences H2A.Z-dependent and -independent gene expression and links ribosomal protein genes to nuclear pores.

• DOI: 10.1371/journal.pgen.1000910
• 发表时间: 2010-04-15
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Yoshida T;Shimada K;Oma Y;Kalck V;Akimura K;Taddei A;Iwahashi H;Kugou K;Ohta K;Gasser SM;Harata M
• 通讯作者: Harata M

Cell-cycle control of meiotic recombination initiation

减数分裂重组起始的细胞周期控制

• 发表时间: 2008
• 作者: 太田邦史;ら
• 通讯作者: ら

Dynamic distribution of meiotic chromosomal proteins along yeast chromosomes

减数分裂染色体蛋白沿酵母染色体的动态分布

• 发表时间: 2007
• 作者: 太田邦史;ら
• 通讯作者: ら

ADLib法による迅速モノクローナル抗体作製

使用 ADLib 方法快速生产单克隆抗体

• 发表时间: 2006
• 作者: 太田邦史;瀬尾秀宗
• 通讯作者: 瀬尾秀宗

Ohta K. Stepwise chromatin remodeling by a cascade of transcription initiation of non-coding RNAs.

Ohta K. 通过非编码 RNA 的转录起始级联进行逐步染色质重塑。

• 发表时间: 2008
• 期刊: Nature 456
• 作者: Hirota K.;Miyoshi T.;Kugou K.;Hoffman C.S.;Shibata T.
• 通讯作者: Shibata T.