Interplay among recombination, replication, segregation, and chromatin structure.

减数分裂重组对遗传多样性的建立和配子的形成起着至关重要的作用。减数分裂重组的调控涉及多种与DNA相关的过程。本项目发现，减数分裂重组启动子Spo11是通过CDC7-Dbf4激酶磷酸化Spo11-Accessspry蛋白Mer2激活的，而CDC7-Dbf4激酶是DNA复制周期的主要调节因子。我们还发现，Spo11的分布受减数分裂染色单体凝聚蛋白Rec8和复制的调节。此外，我们还发现了几个参与染色质修饰的因素，这些因素是激活减数分裂重组热点所必需的。最后，我们发现了一类新的参与裂解酵母应激诱导基因激活的染色质调控的长mRNA型非编码RNA。

Meiotic recombination plays crucial roles in establishment of genetic diversity and gametes formation. The regulation of meiotic recombination involves various DNA-related processes. This project uncovered that meiotic recombination initiator Spo11 is activated by phosphorylation of a Spo11-accesspry protein Mer2 by Cdc7-Dbf4 kinase, which is a master regulator of DNA replication cycle. We also discovered that Spo11 distribution is regulated by meiotic chromatid cohesion protein Rec8 and replication. In addition, we found several factors involved in chromatin modification required for the activation of meiotic recombination hotspots. Finally, we discovered a new class of long mRNA-type non-coding RNA involved in chromatin control for the stress-induced gene activation in fission yeast.