Chromatin regulation by lncRNA

我们以前发现了一个lncRNA参与染色质和基因调控的裂殖酵母fbp1基因。这种lncRNA（mlonRNA，代谢应激诱导的lncRNA）从fbp 1基因的远上游转录，与编码片段重叠。在葡萄糖饥饿的反应中，mlonRNA的级联转录导致fbp1启动子的逐步染色质重构，随后大量的mRNA被诱导。染色质结合Atf1，这是必要的mlonRNA转录，增强mlonRNA转录，从而形成一个正反馈环。我们还发现，mlonRNA被转运到细胞内结合多聚核糖体。由于mlonRNA含有许多uORF，mlonRNA不具有很少的编码潜力。RNA测序分析显示42个mlonRNA样lncRNA以及反义lncRNA。我们还在小鼠培养细胞中检测到mlonRNA样lncRNA。

We previously identified a lncRNA involved in chromatin and gene regulation of fission yeast fbp1 gene. This lncRNA (mlonRNA, metabolic stress induced lncRNA) is transcribed from the far upstream of fbp1 gene overlapping with the coding segment. In response to glucose starvation, cascade transcription of mlonRNAs occur to cause stepwise chromatin remodeling in the fbp1 promoter, followed by massive induction of mRNA.We here revealed histone modification is locally modulated by mlonRNA transcription and some chromatin modifying proteins. Chromatin binding of Atf1, which is necessary for mlonRNA transcription, is enhanced by mlonRNA transcription, thereby forming a positive feedback loop. We also discovered that mlonRNA is transported into cytoplasms to bind polysomes. Since mlonRNA harbors many uORFs, mlonRNA does not have little coding potential. RNA Seq analysis revealed 42 mlonRNA-like lncRNAs as well as antisense lncRNAs. We also detected mlonRNA-like lncRNA in mice cultured cells.