Chromatin regulation by lncRNA

lncRNA对染色质的调控

• 批准号: 21241046
• 负责人: OHTA Kunihiro
• 金额: $ 29.2万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21241046/
• 关键词: ノンコーディングRNA; クロマチン; ヒストン修飾; 転写制御; ゲノム発現; 翻訳制御; エピゲノム; 転写; ストレス応答; グルコース代謝; エビゲノム; ヒストン

We previously identified a lncRNA involved in chromatin and gene regulation of fission yeast fbp1 gene. This lncRNA (mlonRNA, metabolic stress induced lncRNA) is transcribed from the far upstream of fbp1 gene overlapping with the coding segment. In response to glucose starvation, cascade transcription of mlonRNAs occur to cause stepwise chromatin remodeling in the fbp1 promoter, followed by massive induction of mRNA.We here revealed histone modification is locally modulated by mlonRNA transcription and some chromatin modifying proteins. Chromatin binding of Atf1, which is necessary for mlonRNA transcription, is enhanced by mlonRNA transcription, thereby forming a positive feedback loop. We also discovered that mlonRNA is transported into cytoplasms to bind polysomes. Since mlonRNA harbors many uORFs, mlonRNA does not have little coding potential. RNA Seq analysis revealed 42 mlonRNA-like lncRNAs as well as antisense lncRNAs. We also detected mlonRNA-like lncRNA in mice cultured cells.

我们之前发现了一个参与裂变酵母fbp1基因染色质和基因调控的lncRNA。这个lncRNA （mlonRNA，代谢应激诱导的lncRNA）是从fbp1基因的远上游与编码片段重叠的地方转录的。在葡萄糖饥饿的情况下，mlonrna的级联转录导致fbp1启动子中的染色质逐步重塑，随后大量诱导mRNA。我们在这里发现组蛋白修饰是由mlonRNA转录和一些染色质修饰蛋白局部调节的。Atf1的染色质结合是mlonRNA转录所必需的，mlonRNA转录增强了Atf1的染色质结合，从而形成一个正反馈回路。我们还发现mlonRNA被转运到细胞质中结合多聚体。由于mlonRNA含有许多uorf，因此mlonRNA具有不小的编码潜力。RNA Seq分析显示了4200万个类似lncrna的lncrna以及反义lncrna。我们还在小鼠培养细胞中检测到mlonrna样lncRNA。

项目成果

Molecular assembly of meiotic recombination-initiating proteins in fission yeast

裂殖酵母中减数分裂重组起始蛋白的分子组装

• 发表时间: 2010
• 作者: Tanaka;K.;Kurosawa K.;宇山智彦;Ohta K.
• 通讯作者: Ohta K.

Ahistone modification pattern around ATF/CREB-dependent meiotic recombination hotspots in fission yeast

裂殖酵母中 ATF/CREB ​​依赖性减数分裂重组热点周围的组蛋白修饰模式

• 发表时间: 2010
• 作者: Kashida;H.; Asanuma;H;"DNA Conjugates and Sensors" Eds. by K. R. Fox;T. Brown;山根聡;M.Nakaoka;市川隆一;池田祐子;山中幸;高橋照彦;Yamada T.
• 通讯作者: Yamada T.

Massive genome restructuring induced by conditional multiple DNA breaks

条件性多重 DNA 断裂诱导大规模基因组重组

• 发表时间: 2013
• 作者: 水沼佳奈;松木田聖士;不破春彦;佐々木誠;Kunihiro Ohta
• 通讯作者: Kunihiro Ohta

The transcriptiome analysis of stress responsive long non-coding RNAs using directional RNA-seq in fission yeast

使用定向 RNA-seq 对裂殖酵母中的应激响应长非编码 RNA 进行转录组分析

• 发表时间: 2012
• 作者: 小田 有沙;他
• 通讯作者: 他

Transcriptional regulation by glucose starvation stress-induced lncRNAs in yeast

酵母中葡萄糖饥饿应激诱导的 lncRNA 的转录调控

• 发表时间: 2014
• 作者: Oda A.;Takemata N.;Miyoshi T.;Suzuki Y.;Sugano S.;Hoffman C. and Ohta K.
• 通讯作者: Hoffman C. and Ohta K.