Insights into lipopolysaccharide (LPS) transport by solid-state NMR spectroscopy (P01)

脂多糖（LPS）是革兰氏阴性菌不对称外膜的主要成分，并保护它们免受抗生素和哺乳动物免疫系统的侵害。维持这种不对称性的一个重要步骤是核心-LPS通过ABC floppase MsbA在内膜上的易位，LPS通过脂多糖转运（LPT）系统向外膜转移。在P01项目中，我们将通过固态核磁共振研究LPS的转运机制，并探索其存在如何改变膜的性质。

Lipopolysaccharides (LPS) are the main component of the asymmetric outer membrane of Gram-negative bacteria and protect them against antibiotics and the mammalian immune system. One essential step in maintaining this asymmetry is the translocation of core-LPS across the inner membrane by the ABC floppase MsbA from where LPS is carried on towards the outer membrane by the lipopolysaccharide transport (LPT) system. In project P01, we will address the LPS transport mechanism and explore how its presence alters membrane properties by solid-state NMR.