Insights into lipopolysaccharide (LPS) transport by solid-state NMR spectroscopy (P01)

通过固态核磁共振波谱深入了解脂多糖 (LPS) 运输 (P01)

• 批准号: 501342221
• 金额: --
• 依托单位: Institut für Biophysikalische Chemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/501342221?language=en
• 关键词: Insights; into; lipopolysaccharide; LPS; transport

Lipopolysaccharides (LPS) are the main component of the asymmetric outer membrane of Gram-negative bacteria and protect them against antibiotics and the mammalian immune system. One essential step in maintaining this asymmetry is the translocation of core-LPS across the inner membrane by the ABC floppase MsbA from where LPS is carried on towards the outer membrane by the lipopolysaccharide transport (LPT) system. In project P01, we will address the LPS transport mechanism and explore how its presence alters membrane properties by solid-state NMR.

脂多糖（LPS）是革兰氏阴性菌不对称外膜的主要成分，并保护它们免受抗生素和哺乳动物免疫系统的侵害。维持这种不对称性的一个重要步骤是核心-LPS通过ABC翻转酶MsbA穿过内膜的易位，LPS从此处通过脂多糖转运（LPT）系统被携带朝向外膜。在项目P01中，我们将通过固态NMR解决LPS转运机制并探索其存在如何改变膜特性。