Challenging Exploratory Study of Odor Recorder Using Olfactory Receptors

使用嗅觉感受器的气味记录仪的挑战性探索性研究

• 批准号: 24656253
• 负责人: NAKAMOTO Takamichi
• 金额: $ 2.58万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24656253/
• 关键词: センシングデバイス; 嗅覚受容体; 匂いの記録再生; 匂いバイオセンサ; 蛍光測定; 選択性

The fundamental study of an odor recorder using olfactory receptors of an insect was performed. Especially, sensor devices are important since they influence the performance of the odor recorder very much. We used the olfactory receptors of the insect since their mechanism is much simpler than that of a vertebrate. In this study, we established the cell systems with the stable expressions of five olfactory receptors by transferring drosophila olfactory-receptor gene into Sf21 cell together with the gene of fluorescent protein sensitive to calcium, followed by the antibiotic screening. The optical imaging reveals that the odor concentration can be measured using fluorescent response of each cell system to odorant. Thus, it was found that the established cell system can be used as a sensor component.Then, we studied the simple fluorescent measurement system. We fabricated the measurement cell, where a power LED illuminated the cells and their fluorescence was detected by a photo de via an optical filter. Although we performed the experiments on fluorescent beads and the cells, it was found that the light intensity from the power LED was insufficient. However, the large fluorescent intensity was obtained when we used a laser and improved the illumination method. Moreover, we investigated the feasibility of odor approximation by blending several odor components by simulation when we used the responses of multiple olfactory receptors to odorants accumulated in the database.

对利用昆虫嗅觉感受器的气味记录仪进行了基础性研究。特别是，传感器装置是重要的，因为它们非常影响气味记录器的性能。我们使用了昆虫的嗅觉受体，因为它们的机制比脊椎动物简单得多。本研究通过将果蝇嗅觉受体基因与钙敏感荧光蛋白基因一起转入Sf 21细胞，并进行抗生素筛选，建立了5种嗅觉受体稳定表达的细胞系。光学成像揭示了气味浓度可以使用每个细胞系统对气味剂的荧光响应来测量。因此，我们发现所建立的细胞系统可以作为一个传感器组件。然后，我们研究了简单的荧光测量系统。我们制作了测量池，其中功率LED照亮细胞，并且它们的荧光通过经由滤光器的光电二极管检测。虽然我们对荧光珠和细胞进行了实验，但发现功率LED的光强度不足。然而，当我们使用激光和改进的照明方法时，获得了大的荧光强度。此外，我们研究了气味近似的可行性，通过混合几种气味成分的模拟时，我们使用的多个嗅觉受体的气味在数据库中积累的反应。

项目成果

小さくても強大な能力・昆虫脳の秘密に迫る!

特写昆虫大脑的秘密，小而强大的能力！

• 发表时间: 2012
• 作者: 神崎 亮平;オーム社
• 通讯作者: オーム社

"Fundamentals of odor recorder" in Human Olfactory Displays and Interfaces: Odor Sensing and Presentation

人类嗅觉显示和界面中的“气味记录器基础知识：气味感知和呈现”

• 发表时间: 2012
• 作者: 斉藤保典;小林一樹;Takamichi Nakamoto
• 通讯作者: Takamichi Nakamoto

匂い結合タンパク質を利用した匂い物質可溶化技術の開発

开发利用气味结合蛋白的气味溶解技术

• 发表时间: 2013
• 作者: 三觜裕之;櫻井健志;藤井毅;光野秀文;石川幸男;神崎亮平
• 通讯作者: 神崎亮平

"Fundamentals of odor recorder" in Human Olfactory Displays and Interfaces

人类嗅觉显示和界面中的“气味记录器基础知识”

• 发表时间: 2012
• 作者: T.Nakamoto;IGI Global
• 通讯作者: IGI Global

昆虫の嗅覚受容体を再現した高機能な匂 いセンサの開発

开发可复制昆虫嗅觉受体的高性能气味传感器

• 发表时间: 2012
• 作者: R. Sivakumar;T. Tsunoda;Y. Kuroki;T. Okamoto and M. Takata;光野 秀文,神崎 亮平
• 通讯作者: 光野 秀文,神崎 亮平