Challenging Exploratory Study of Odor Recorder Using Olfactory Receptors

的基本研究昆虫的气味记录器使用嗅觉受体。其中，传感器对气味记录仪的性能影响很大。我们使用昆虫的嗅觉感受器，因为它们的机制比脊椎动物简单得多。本研究通过将果蝇嗅觉受体基因与钙敏感荧光蛋白基因一起转入Sf21细胞，建立了5种嗅觉受体稳定表达的细胞体系，并进行抗生素筛选。光学成像表明，可以利用每个细胞系统对气味的荧光响应来测量气味浓度。因此，发现所建立的细胞系统可以用作传感器组件。然后，我们研究了简单的荧光测量系统。我们制作了测量细胞，其中电源LED照亮细胞，并通过光学滤光片通过照片检测其荧光。虽然我们对荧光珠和细胞进行了实验，但发现功率LED的光强不足。然而，当我们使用激光并改进照明方法时，获得了较大的荧光强度。此外，我们利用数据库中积累的多个嗅觉受体对气味的反应，通过模拟研究了混合几种气味成分的气味近似的可行性。

The fundamental study of an odor recorder using olfactory receptors of an insect was performed. Especially, sensor devices are important since they influence the performance of the odor recorder very much. We used the olfactory receptors of the insect since their mechanism is much simpler than that of a vertebrate. In this study, we established the cell systems with the stable expressions of five olfactory receptors by transferring drosophila olfactory-receptor gene into Sf21 cell together with the gene of fluorescent protein sensitive to calcium, followed by the antibiotic screening. The optical imaging reveals that the odor concentration can be measured using fluorescent response of each cell system to odorant. Thus, it was found that the established cell system can be used as a sensor component.Then, we studied the simple fluorescent measurement system. We fabricated the measurement cell, where a power LED illuminated the cells and their fluorescence was detected by a photo de via an optical filter. Although we performed the experiments on fluorescent beads and the cells, it was found that the light intensity from the power LED was insufficient. However, the large fluorescent intensity was obtained when we used a laser and improved the illumination method. Moreover, we investigated the feasibility of odor approximation by blending several odor components by simulation when we used the responses of multiple olfactory receptors to odorants accumulated in the database.