Mass spectrometer with MALDI2 and ESI ion source,Laser Capture Microdissection device

配备 MALDI2 和 ESI 离子源的质谱仪、激光捕获显微切割装置

• 批准号: 504501554
• 金额: --
• 依托单位: Institut für Experimentelle Immunologie und Bildgebung
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/504501554?language=en
• 关键词: Mass; spectrometer; MALDI2; ESI; ion

Imaging modalities have the potential to decipher the distribution of immune cells and the local microenvironment in tissues. The registration of imaging datasets could be the key to comprehensively unravelling the cell and tissue specific mechanisms in inflammatory processes in the future. At IMCES, state-of-the-art microscopes are already being used to determine the distribution of immune cells. By establishing local mass spectrometry in the context of this large-scale equipment application, it will be possible not only to determine the distribution of immune cells in tissues, but also to register these distribution patterns with the local proteome. Thus, novel insights of the local molecular mechanisms that mediate leukocyte infiltration and inflammatory conditions in diseases will be provided. The selection of project leaders with complementary expertise in immunology, infectious diseases, neurology, cancer and endocrinology will allow unraveling disease-, organ-, and cell-specific mechanisms in various diseases. The corresponding research topics are currently being addressed by the project leaders in existing and proposed DFG consortia and represent the current research focus of the Medical Faculty of the University of Duisburg-Essen. The expansion of the imaging capabilities in Essen via local mass spectrometry represents a consistent further development of state-of-the-art imaging modalities at IMCES.

成像方式有可能破译免疫细胞的分布和组织中的局部微环境。成像数据集的配准可能是未来全面揭示炎症过程中细胞和组织特异性机制的关键。在IMCES，最先进的显微镜已经被用于确定免疫细胞的分布。通过在这种大规模设备应用的背景下建立局部质谱，不仅可以确定免疫细胞在组织中的分布，还可以将这些分布模式与局部蛋白质组进行登记。因此，新的见解的局部分子机制，介导白细胞浸润和炎症条件的疾病将提供。选择在免疫学，传染病，神经学，癌症和内分泌学方面具有互补专业知识的项目负责人将有助于解开各种疾病的疾病，器官和细胞特异性机制。相应的研究课题目前正在解决的项目领导人在现有的和拟议的DFG财团，并代表了杜伊斯堡-埃森大学医学院目前的研究重点。埃森通过当地质谱法扩大成像能力代表了IMCES最先进成像模式的进一步发展。