Elektrospray-Triple-Quadrupole-/QTRAP Tandem Mass Spectrometer (UHPLC-ESI-TQ/QTRAP-MS/MS)

电喷雾三重四极杆/QTRAP 串联质谱仪 (UHPLC-ESI-TQ/QTRAP-MS/MS)

• 批准号: 504503897
• 金额: --
• 依托单位: Universität Hohenheim
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/504503897?language=en
• 关键词: Elektrospray; Triple; Quadrupole; QTRAP; Tandem

A system consisting of an ultra-high-performance liquid chromatography system and an electrospray triple quadrupole/QTRAP mass spectrometer (UHPLC-ESI-TQ / QTRAP-MS/MS) shall be procured. The UHPLC system should include a binary high-pressure pump with a pressure limit of 1200 bar, a heated column oven and a thermostated autosampler. The TQ/QTRAP mass spectrometer should have high selectivity as well as high sensitivity for the detection of metabolites in MRM (Multiple Reaction Monitoring) mode to enable targeted quantitative analysis of low-abundant metabolites and to avoid interferences with matrix background. As additional options to optimize the separation of target analytes from matrix background and isobaric compounds, the TQ/QTRAP mass spectrometer should have an upstream ion mobility and the option to use a second generation of productions for quantification (MRM3). Furthermore, the possibility of tandem mass spectrometry (MS/MS) should be given within an MRM experiment to confirm the identity of target analytes. In addition, powerful software tools for processing and interpreting the mass spectrometry data are an important part of the overall system.The system applied for is intended to be used for the targeted quantitative analysis of small organic molecules in complex biological samples. One focus is the targeted quantitative analysis of phytohormones in plant model organisms and cultivated plants. On one hand, the results should lead to a better understanding of the signalling pathways that are involved in the adaptation of these plants to different biotic interactions and, on the other hand, it should be elucidated how plants integrate different environmental signals that are associated with stress. Furthermore, the role of phytohormones and other substances (saponins, lipids and flavonoids) in cultivated plants in relation to abiotic stress factors such as salt content in the soil, water deficiency, heat or cold will be investigated. The aim is to gain knowledge of plant signalling pathways and adaptation mechanisms, which should lead to more efficient cultivation of crops.Furthermore, the system applied for is intended to be used for is the quantitative analysis of primary and secondary metabolites in the blood, milk and the intestinal contents of farm animals (cows, chickens, pigs). The aim of these investigations is to characterize the effects of feeding, husbandry and management on the metabolism and the microbiome of livestock and thereby identify biomarkers for the metabolic health of livestock. These results should form the basis for resource-saving and animal welfare food production.

应采购一个由超高性能液相色谱系统和电喷雾三重四极杆/QTRAP质谱仪(UHPLC-ESI-TQ/QTRAP-MS/MS)组成的系统。UHPLC系统应包括一个压力极限为1200 bar的二元高压泵、一个加热柱烘箱和一个恒温自动进样器。TQ/QTRAP质谱仪对于MRM(多反应监测)模式下的代谢物检测应具有高选择性和高灵敏度，以实现对低丰度代谢物的有针对性的定量分析，并避免与基质背景的干扰。作为优化目标分析物与基质背景和同量异压化合物分离的附加选项，TQ/QTRAP质谱仪应具有上游离子迁移率，并可选择使用第二代产物进行定量(MRM3)。此外，串联质谱仪(MS/MS)的可能性应在MRM实验中提供，以确认目标分析物的身份。此外，功能强大的软件工具用于处理和解释质谱学数据是整个系统的重要组成部分，所申请的系统旨在用于复杂生物样品中有机小分子的靶向定量分析。一个焦点是对植物模式生物和栽培植物中的植物激素进行有针对性的定量分析。一方面，这些结果应该有助于更好地理解这些植物适应不同生物相互作用的信号通路，另一方面，应该阐明植物如何整合与胁迫相关的不同环境信号。此外，还将研究栽培植物中的植物激素和其他物质(皂苷、脂类和类黄酮类)与非生物胁迫因素(如土壤含盐量、缺水、热或冷)的作用。其目的是了解植物信号通路和适应机制，从而更有效地种植作物。此外，所申请的系统旨在用于对农场动物(牛、鸡、猪)的血液、牛奶和肠道内容物中的初级和次级代谢物进行定量分析。这些调查的目的是描述饲养、饲养和管理对家畜代谢和微生物群的影响，从而确定家畜代谢健康的生物标志物。这些结果应该成为资源节约型和动物福利食品生产的基础。