Elektrospray-Triple-Quadrupole-/QTRAP Tandem Mass Spectrometer (UHPLC-ESI-TQ/QTRAP-MS/MS)

采购一套由一套超高效液相色谱系统和一台电喷雾三重四极杆/QTRAP质谱仪（UHPLC-ESI-TQ /QTRAP -MS/MS）组成的系统。UHPLC系统应包括一个压力限制为1200 bar的二元高压泵，一个加热柱箱和一个恒温自动进样器。TQ/QTRAP质谱仪在MRM （Multiple Reaction Monitoring，多反应监测）模式下对代谢物的检测应具有高选择性和高灵敏度，能够对低丰度代谢物进行有针对性的定量分析，避免基质背景干扰。作为优化目标分析物与基质背景和等压化合物分离的额外选项，TQ/QTRAP质谱仪应该具有上游离子迁移率，并可选择使用第二代产品进行定量（MRM3）。此外，应该在MRM实验中给出串联质谱（MS/MS）的可能性，以确认目标分析物的身份。此外，用于处理和解释质谱数据的强大软件工具是整个系统的重要组成部分。本系统申请用于复杂生物样品中小有机分子的靶向定量分析。其中一个重点是植物模式生物和栽培植物中植物激素的定向定量分析。一方面，这些结果将有助于更好地理解这些植物适应不同生物相互作用所涉及的信号通路，另一方面，它应该阐明植物如何整合与胁迫相关的不同环境信号。此外，植物激素和其他物质（皂苷、脂类和类黄酮）在栽培植物中与土壤含盐量、缺水、热或冷等非生物胁迫因素有关的作用将被研究。其目的是获得植物信号通路和适应机制的知识，这将导致更有效的作物种植。此外，申请的系统旨在用于农场动物（牛、鸡、猪）血液、牛奶和肠道内容物中的初级和次级代谢物的定量分析。这些调查的目的是表征饲养、饲养和管理对牲畜代谢和微生物组的影响，从而确定牲畜代谢健康的生物标志物。这些结果应该成为资源节约和动物福利食品生产的基础。

A system consisting of an ultra-high-performance liquid chromatography system and an electrospray triple quadrupole/QTRAP mass spectrometer (UHPLC-ESI-TQ / QTRAP-MS/MS) shall be procured. The UHPLC system should include a binary high-pressure pump with a pressure limit of 1200 bar, a heated column oven and a thermostated autosampler. The TQ/QTRAP mass spectrometer should have high selectivity as well as high sensitivity for the detection of metabolites in MRM (Multiple Reaction Monitoring) mode to enable targeted quantitative analysis of low-abundant metabolites and to avoid interferences with matrix background. As additional options to optimize the separation of target analytes from matrix background and isobaric compounds, the TQ/QTRAP mass spectrometer should have an upstream ion mobility and the option to use a second generation of productions for quantification (MRM3). Furthermore, the possibility of tandem mass spectrometry (MS/MS) should be given within an MRM experiment to confirm the identity of target analytes. In addition, powerful software tools for processing and interpreting the mass spectrometry data are an important part of the overall system.The system applied for is intended to be used for the targeted quantitative analysis of small organic molecules in complex biological samples. One focus is the targeted quantitative analysis of phytohormones in plant model organisms and cultivated plants. On one hand, the results should lead to a better understanding of the signalling pathways that are involved in the adaptation of these plants to different biotic interactions and, on the other hand, it should be elucidated how plants integrate different environmental signals that are associated with stress. Furthermore, the role of phytohormones and other substances (saponins, lipids and flavonoids) in cultivated plants in relation to abiotic stress factors such as salt content in the soil, water deficiency, heat or cold will be investigated. The aim is to gain knowledge of plant signalling pathways and adaptation mechanisms, which should lead to more efficient cultivation of crops.Furthermore, the system applied for is intended to be used for is the quantitative analysis of primary and secondary metabolites in the blood, milk and the intestinal contents of farm animals (cows, chickens, pigs). The aim of these investigations is to characterize the effects of feeding, husbandry and management on the metabolism and the microbiome of livestock and thereby identify biomarkers for the metabolic health of livestock. These results should form the basis for resource-saving and animal welfare food production.