Triple Quadrupole-Mass Spectrometer

我们正在申请最新一代的三重四极杆质谱仪。预期在科隆大学“卓越集群：衰老相关疾病中的细胞应激反应”（CECAD）的中央“脂质组学/代谢组学核心设施”中使用，并将安装在CECAD研究中心设施的实验室中。在那里，该设施将把分光计用于科隆大学用户团体正在进行和计划进行的项目，也用于外部学术研究团体的项目。从长远来看，新的质谱仪将取代已有10年历史的前身型号，该设施已非常成功地用于生物样品中脂质的目标定量。该设施的服务分析最近扩大到包括极性和离子代谢物的有针对性的定量，导致对复杂生物样品（如各种模型生物体、细胞、组织、体液和分离的细胞器）中的小代谢物分析的需求大幅增加。与其前身相比，所要求的质谱仪的特点是不仅在正离子模式下而且在负离子模式下都具有极高的灵敏度。这对于极性和离子代谢物的检测至关重要，与大多数脂质物质相比，极性和离子代谢物通常必须在负离子模式下测量，并且以非常低的浓度存在于生物样品中。所要求的光谱仪的快速极性切换能力允许同时检测脂质和极性代谢物。此外，该检测器的大线性动态范围可通过单次进样对低浓度代谢物和含量非常丰富的化合物进行稳健的定量。这对于生物样品中大量代谢物的高通量定量至关重要，其中材料的量通常非常有限。总体而言，所要求的质谱仪最佳地满足了复杂生物样品中小代谢物的目标定量仪器的要求，并将使CECAD脂质组学/代谢组学设施能够满足对定量代谢组学分析日益增长的需求。

We are applying for a triple quadrupole-mass spectrometer of the latest generation. It is intended for use in the central "Lipidomics/Metabolomics Core Facility" of the "Cluster of Excellence: Cellular Stress Responses in Aging-Associated Diseases" (CECAD) at the University of Cologne and will be installed in a laboratory of the Facility in the CECAD Research Center. There, the Facility will use the spectrometer for ongoing and planned projects of its user groups at the University of Cologne, but also for projects of external academic research groups. In the long term, the new mass spectrometer will replace its 10-year-old predecessor model, which the Facility has used very successfully for targeted quantifications of lipids in biological samples. The recent expansion of the Facility's service analyses to include targeted quantification of polar and ionic metabolites has led to a strong increase in demand for small metabolite analyses in complex biological samples such as various model organisms, cells, tissues, body fluids, and isolated cell organelles. Compared to its predecessor, the requested mass spectrometer is characterized by its extremely high sensitivity not only in the positive but also in the negative ion mode. This is crucial for the detection of polar and ionic metabolites, which, in contrast to most lipid species, usually have to be measured in the negative ion mode and are present in biological samples in very low concentrations. The fast polarity switching capabilities of the requested spectrometer allows simultaneous detection of lipids and polar metabolites. In addition, the large linear dynamic range of the detector enables robust quantification of both, metabolites at low concentrations and very abundant compounds with a single sample injection. This is critical for high throughput quantifications of large numbers of metabolites in biological samples, where the amount of material is typically very limited. Overall, the requested mass spectrometer optimally fulfils the requirements for an instrument for the targeted quantification of small metabolites in complex biological samples and will enable the CECAD Lipidomics/Metabolomics Facility to meet the increasing demand for quantitative metabolomics analyses.