Triple quadrupole linear ion trap mass spectrometer coupled to a HPLC system

与 HPLC 系统联用的三重四极杆线性离子阱质谱仪

• 批准号: 505943254
• 金额: --
• 依托单位: Universität Rostock
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/505943254?language=en
• 关键词: Triple; quadrupole; linear; ion; trap

The proposed triple quadrupole linear ion trap mass spectrometer coupled to a high-pressure liquid chromatography system (HPLC-MS/MS) represents a device system for the highly specific and highly sensitive analysis of small organic molecules (< 2000 Da). The device should on the one hand enable quantification of lowest concentrations of drugs and their metabolites, as well as endogenous substances (biomarkers) in complex biological matrices (e.g. serum, organ homogenates, cell lysates). On the other hand, the device system should be used for the identification and quantification of proteins such as drug transporters and metabolizing enzymes in in vitro systems (e.g. cell lines, microsomes, organoids) and human and animal tissue samples using the so-called targeted proteomics approach.For the presented aims, the device applied for represents the optimal research platform, as it combines the highest specificity with the highest measurement sensitivity. In addition, combined experiments can be carried out that previously could only be carried out with two different types of mass spectrometers (i.e., ion trap mass spectrometer and triple quadrupole mass spectrometer). The overall research goal of the applying working group is the characterization of the expression, regulation and function of metabolizing enzymes and transport proteins, which are of great relevance for the pharmacokinetics, efficacy and safety of drug therapy. The device system applied for and the associated combination of the highest measurement sensitivity and specificity is an essential prerequisite for the realization of the research goals set. The Institute for Pharmacology and Toxicology at the Rostock University Medical Center already has extensive experience in the field of LC-MS/MS analysis and also has the appropriate equipment. However, the existing old devices are on the one hand heavily used and on the other hand not sufficiently sensitive or technically not suitable to be able to address the current issues. The requested device would enable us to carry out our own research analyzes with previously unimagined sensitivity and specificity.In addition to realizing your own research questions, the device system is also used within the framework of university-internal, as well as national and international cooperation. The main goal here is on the one hand the establishment of stable and long-term research cooperations and on the other hand a sustainable scientific exploitation in the form of high-ranking publications and, if possible, also patent applications.

提出的三倍四极线离子陷阱质谱仪与高压液相色谱系统（HPLC-MS/MS）耦合，代表了用于对小有机分子（<2000 DA）的高度特异性且高度敏感的分析的设备系统。该装置应一方面能够定量最低浓度的药物及其代谢产物，以及复杂生物基质（例如血清，器官匀浆，细胞裂解物）中的内源性物质（生物标志物）。另一方面，应将设备系统用于鉴定和定量蛋白质（例如药物转运蛋白和代谢酶）在体外系统（例如细胞系，微粒体，器官）和人类和动物组织样品中使用所谓的靶向蛋白质组学方法的最高工具的最高效率来指示，该工具是指该的最高效率。另外，可以进行合并的实验，以前只能使用两种不同类型的质谱仪（即离子陷阱质谱仪和三倍四极杆质谱仪）进行。应用工作组的总体研究目标是代谢酶和转运蛋白的表达，调控和功能的表征，这与药物治疗的药代动力学，功效和安全性非常相关。应用的设备系统以及最高测量灵敏度和特异性的相关组合是实现研究目标集的必要先决条件。罗斯托克大学医学中心的药理学和毒理学研究所已经在LC-MS/MS分析领域拥有丰富的经验，并且具有适当的设备。但是，现有的旧设备一方面是大量使用的，另一方面不够敏感或技术上不适合解决当前问题。要求的设备将使我们能够以先前没有想象的灵敏度和特异性进行自己的研究分析。除了意识到自己的研究问题外，该设备系统还在大学内部以及国家和国际合作的框架内使用。这里的主要目标是建立稳定和长期的研究合作，另一方面，以高级出版物的形式进行可持续的科学剥削，并在可能的情况下，也是专利申请。