Iron toxicity in Chlamydomonas in relation to P limitation

衣藻中铁毒性与磷限制相关

• 批准号: 50458766
• 负责人: Privatdozentin Dr. Elly Spijkerman
• 金额: --
• 依托单位: Arbeitsgruppe Ökologie/Ökosystemmodellierung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/50458766?language=en
• 关键词: Iron; toxicity; Chlamydomonas; relation; limitation

The green flagellate algal species Chlamydomonas acidophila faces co-limiting conditions in a multi-stress environment. In this proposal, co-limiting conditions for inorganic P (Pi) and inorganic carbon (CO2) as well as survival in lakes with high iron concentrations (multi-stress) are the central topics.In the very acidic lakes, growth of C. acidophila is potentially limited by Pi and/or CO2. In ongoing laboratory experiments, a trade-off in the acquisition of either Pi or CO2 was revealed, that was more strongly related to the cellular P quota (Qp) than to the external nutrient concentration. This correlation enhanced our understanding of the dynamics of natural phytoplankton populations possibly co-limited for Pi and CO2. First data show a high tolerance for high iron concentrations on the growth and photosynthetic yield of C. acidophila, but also negative effects. For example, high extemal Fe concentrations resulted in a cellular Pi-limitation. Other results showed a negative correlation between enhanced cellular Fe accumulations and the chlorophyll content. In a 3rd year of the project GA401/12, I want to finalise the experimental work on a co-limitation, and study the physiological response (e.g. Fe accumulation and location, C, P and Fe cell quotas and photosynthesis) of C acidophila to different ambient P:Fe ratios with the aim to unravel mechanisms of iron tolerance and the impact of Fe accumulation on the ecophysiology of our model algal species.

绿色鞭毛藻种类衣藻嗜酸在多重胁迫环境中面临共同限制条件。在这个方案中，无机磷(PI)和无机碳(CO2)的共同限制条件以及在铁浓度较高的湖泊(多重胁迫)中的生存是中心议题。在极酸性的湖泊中，嗜酸梭菌的生长潜在地受到PI和/或CO2的限制。在正在进行的实验室实验中，PI或CO2的获取被揭示出一种权衡，这与细胞P配额(QP)比外部营养浓度更密切相关。这种相关性增强了我们对自然浮游植物种群动态的理解，这些种群可能是PI和CO2共同限制的。第一个数据表明，高铁浓度对嗜酸乳杆菌的生长和光合作用产量有很高的耐受性，但也有负面影响。例如，较高的外源铁浓度导致了细胞内等电点的限制。其他结果表明，细胞铁积累增加与叶绿素含量呈负相关。在GA401/12项目的第三年，我想完成共同限制的实验工作，并研究嗜酸梭菌对不同环境P：Fe比率的生理响应(例如，Fe的积累和位置、C、P和Fe细胞配额以及光合作用)，目的是揭示耐铁性的机制，以及Fe积累对我们的模式藻类生态生理的影响。