The role of late endolysosomal transport in podocyte functions.

晚期内溶酶体转运在足细胞功能中的作用。

• 批准号: 505273162
• 负责人: Professor Dr. Hermann Pavenstädt
• 金额: --
• 依托单位: Medizinische Klinik D: Allg. Innere Medizin und Notaufnahme sowie Nieren- und Hochdruckkrankheiten und Rheumatologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/505273162?language=en
• 关键词: role; late; endolysosomal; transport; podocyte

Disturbed balance between endo- and autophagocytosis plays an important role in the pathogenesis of renal diseases. Our hypothesis is that the late endolysosomal transport/degradation pathway has specific functions for podocytes. To test this hypothesis, we knocked down the GTPase Rab7, a key enzyme of late endolysosomal and autolysosomal transport, in mouse podocytes in vivo. To test this hypothesis, we knocked down the GTPase Rab7, a key enzyme of late endolysosomal and autolysosomal transport, in mouse podocytes in vivo. Mice with a podocyte-specific knockout of Rab7 developed podocyte injury and became proteinuric. Using cell biology techniques, we aim to understand the mechanisms of this podocyte injury; in particular, we aim to investigate podocyte functions and signaling pathways, their endolysosomal transport including lysosome function after knockdown of Rab7. The project will provide important insights into the function of late endolysosomal transport for podocyte function.

自噬与内噬失衡在肾脏疾病的发病机制中起着重要作用。我们的假设是，晚期内溶酶体转运/降解途径对足细胞具有特定的功能。为了验证这一假设，我们敲低了小鼠足细胞中晚期内溶酶体和自体溶酶体转运的关键酶GTTRab 7。为了验证这一假设，我们敲低了小鼠足细胞中晚期内溶酶体和自体溶酶体转运的关键酶GTTRab 7。足细胞特异性敲除Rab 7的小鼠出现足细胞损伤并出现蛋白尿。使用细胞生物学技术，我们的目标是了解这种足细胞损伤的机制，特别是，我们的目标是研究足细胞的功能和信号通路，其内溶酶体转运，包括溶酶体功能后敲低Rab 7。该项目将提供重要的洞察力，为足细胞功能的晚期内溶酶体转运的功能。