Role of the Arabidopsis TRAPPII tethering complex in division plane establishment

拟南芥 TRAPPII 系链复合体在划分平面建立中的作用

• 批准号: 505695129
• 负责人: Professorin Dr. Farhah Assaad, Ph.D.
• 金额: --
• 依托单位: Professur für Biotechnologie der Naturstoffe (BiNa)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/505695129?language=en
• 关键词: Role; Arabidopsis; TRAPPII; tethering; complex

In plants, the position of the interphase nucleus determines the division plane prior to mitotic entry. Later in the cell cycle, nascent cross walls are assembled in a transient membrane compartment referred to as the cell plate. The Arabidopsis transport protein particle TRAPPII tethering complex is required for both division plane establishment and cell plate formation. We have screened for conditional protein-protein interactions using TRAPPII as bait and have validated interesting candidates. Our preliminary data has led to the working model that TRAPPII is a target of GSK3/shaggy-like kinases (AtSKs) and PP2A phosphatases. We posit that a TRAPPII phosphocode mediates the spatiotemporal control of plant cytokinesis. This grant focuses on division plane establishment and on the TRAPPII-PP2A interaction; PP2A is a component of the TON/TRM/PP2A complex, which is required for cross wall orientation. Our specific aims are to (1) elucidate the role of TRAPPII in division plane establishment, (2) elucidate the role of PP2A in regulating TRAPPII localization and function, (3) establish whether TRAPPII is a PP2A phosphatase substrate. We will combine molecular, genetic, cell biological, biochemical and proteomic approaches to address these goals. The AtSK and PP2A gene families integrate a vast number of biotic and abiotic signals and could thereby instruct trafficking, mobilization and sorting decisions via the TRAPPII phosphocode. In addition to new insights on division plane establishment, this study will more broadly elucidate how, downstream of shaggy-like kinases and PP2A, TRAPPII regulates trafficking in response to environmental and developmental cues.

在植物中，间期核的位置决定了有丝分裂进入之前的分裂平面。在细胞周期的后期，新生的横壁被组装在称为细胞板的瞬态膜室中。拟南芥转运蛋白颗粒 TRAPPII 束缚复合物是分裂平面建立和细胞板形成所必需的。我们使用 TRAPPII 作为诱饵筛选了条件蛋白质-蛋白质相互作用，并验证了有趣的候选者。我们的初步数据得出了 TRAPPII 是 GSK3/shaggy 样激酶 (AtSK) 和 PP2A 磷酸酶的靶标的工作模型。我们假设 TRAPPII 磷酸编码介导植物胞质分裂的时空控制。该资助重点关注划分平面的建立和 TRAPPII-PP2A 交互； PP2A 是 TON/TRM/PP2A 复合体的组成部分，它是跨墙定向所必需的。我们的具体目标是（1）阐明 TRAPPII 在分割平面建立中的作用，（2）阐明 PP2A 在调节 TRAPPII 定位和功能中的作用，（3）确定 TRAPPII 是否是 PP2A 磷酸酶底物。我们将结合分子、遗传、细胞生物学、生化和蛋白质组学方法来实现这些目标。 AtSK 和 PP2A 基因家族整合了大量的生物和非生物信号，从而可以通过 TRAPPII 磷酸代码指导运输、动员和分类决策。除了关于分割平面建立的新见解外，这项研究还将更广泛地阐明在毛状激酶和 PP2A 下游，TRAPPII 如何根据环境和发育线索调节运输。