Role of the Arabidopsis TRAPPII tethering complex in protein sorting at the Trans-Golgi-Network

拟南芥 TRAPPII 系链复合物在跨高尔基体网络蛋白质分选中的作用

• 批准号: 5441912
• 负责人: Professorin Dr. Farhah Assaad, Ph.D.
• 金额: --
• 依托单位: Europäisches Laboratorium für Molekularbiologie (EMBL)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5441912?language=en
• 关键词: Role; Arabidopsis; TRAPPII; tethering; complex

The trans-Golgi network (TGN) is a major hub for the flow and sorting of information from and to the cell surface. The molecular mechanisms underlying protein sorting at the TGN, however, are poorly understood. The focus of this proposal is on the regulation of TGN structure and function in the context of cytokinesis and of light responses. The Arabidopsis TRAPPII tethering complex, thought to function as guanidine exchange factor (GEF) regulating Rab GTPase activity, is emerging as a central player in cytokinesis and in post-Golgi traffic. We have identified interaction partners of the TRAPPII complex via proteomic approaches. We propose to test the role of a subset of these interactors in protein sorting at the cell plate. We will also address rewiring of the TRAPPII proteome in response to environmental stimuli. Our experimental plan integrates molecular genetics, physiology, biochemistry, proteomics, computational and cell biological methods, including super-resolution confocal live imaging microscopy and focused ion beam/scanning electron microscopy, to address TGN structure and function. The outcome of the proposed experiments will considerably further our understanding of the role of the TGN in driving and fine tuning plant growth and adaptation.

跨高尔基体网络(TGN)是细胞表面信息流动和分类的主要枢纽。然而，在TGN进行蛋白质分类的分子机制却知之甚少。这项建议的重点是在胞质分裂和光反应的背景下调节TGN的结构和功能。拟南芥TRAPPII系留复合体被认为是调节Rab GTP酶活性的胍交换因子，正在成为细胞质分裂和高尔基体后运输的中心角色。我们已经通过蛋白质组学的方法确定了TRAPPII复合体的相互作用伙伴。我们建议测试这些相互作用因子的子集在细胞板上的蛋白质分选中的作用。我们还将解决TRAPPII蛋白质组对环境刺激的重新连接问题。我们的实验计划综合了分子遗传学、生理学、生物化学、蛋白质组学、计算和细胞生物学方法，包括超分辨率共聚焦实时成像显微镜和聚焦离子束/扫描电子显微镜，以研究TGN的结构和功能。建议的实验结果将大大加深我们对TGN在驱动和微调植物生长和适应方面的作用的理解。

项目成果

Independent yet overlapping pathways ensure the robustness and responsiveness of trans-Golgi network functions in Arabidopsis

独立但重叠的途径确保拟南芥中跨高尔基体网络功能的稳健性和响应性

• DOI: 10.1242/dev.169201
• 发表时间: 2018
• 期刊: Development
• 影响因子: 4.6
• 作者: Ravikumar R;Kalbfuß N;Gendre D;Steiner A;Altmann M;Altmann S;Rybak K;Edelmann H;Stephan F;Lampe M;Facher E;Wanner G;Falter-Braun P;Bhalerao RP;Assaad FF
• 通讯作者: Assaad FF