Role of the Arabidopsis TRAPPII tethering complex in protein sorting at the Trans-Golgi-Network

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The trans-Golgi network (TGN) is a major hub for the flow and sorting of information from and to the cell surface. The molecular mechanisms underlying protein sorting at the TGN, however, are poorly understood. The focus of this proposal is on the regulation of TGN structure and function in the context of cytokinesis and of light responses. The Arabidopsis TRAPPII tethering complex, thought to function as guanidine exchange factor (GEF) regulating Rab GTPase activity, is emerging as a central player in cytokinesis and in post-Golgi traffic. We have identified interaction partners of the TRAPPII complex via proteomic approaches. We propose to test the role of a subset of these interactors in protein sorting at the cell plate. We will also address rewiring of the TRAPPII proteome in response to environmental stimuli. Our experimental plan integrates molecular genetics, physiology, biochemistry, proteomics, computational and cell biological methods, including super-resolution confocal live imaging microscopy and focused ion beam/scanning electron microscopy, to address TGN structure and function. The outcome of the proposed experiments will considerably further our understanding of the role of the TGN in driving and fine tuning plant growth and adaptation.