Characterization of a core module required for allocation decisions and adaptive responses in Arabidopsis.

拟南芥分配决策和适应性响应所需核心模块的表征。

• 批准号: 428023713
• 负责人: Professorin Dr. Farhah Assaad, Ph.D.
• 金额: --
• 依托单位: Professur für Biotechnologie der Naturstoffe (BiNa)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/428023713?language=en
• 关键词: Characterization; core; module; required; allocation

Adaptive responses can be broken down into four steps: sensing, signal integration, decision making processes and the execution of these decisions. While progress has been made in the field of Arabidopsis to understand signal perception and integration, little is known about decision making processes (here referred to as allocation decisions) and their execution. To address this, we have set up a “conflict of interest” forward genetic screen to identify major players in allocation decisions. Together with reverse genetic approaches and screens for interactors, we have uncovered a three-component module. The first component of the module is the shaggy-like kinase BIN2, which mediates signal integration. The second component is the conserved TRAPPII multi-subunit tethering complex that mediates decision-making processes at the trans-Golgi-network (TGN). The third component comprises a family of Rab GTPases that are posited to execute decisions downstream of the first two components. To dissect the molecular mechanisms of signal convergence at the BIN2-TRAPP-RAB module, we will use a combination of proteomics, biochemistry, genetics and high-resolution confocal microscopy. Our specific goals are to (1) elucidate the impact of brassinosteroid (BR)-induced post-translational modifications on the assembly and function of the TGN-associated TRAPPII tethering complexes, (2) characterize functional interactions between TRAPP complexes and Rab GTPases, and (3) assess how these instruct sorting and trafficking decisions. Our study will identify molecular mechanisms by which signaling at the TGN modulates sorting decisions that contribute to cell division, elongation, growth anisotropy and meristem function. Mechanistic insights gained here will lay a foundation for understanding plant adaptive growth and allocation decisions.

适应性反应可以分为四个步骤：感知，信号整合，决策过程和执行这些决定。虽然在拟南芥领域已经取得了进展，以了解信号的感知和整合，很少有人知道决策过程（这里称为分配决策）及其执行。为了解决这个问题，我们建立了一个“利益冲突”的遗传筛查，以确定分配决策的主要参与者。再加上反向遗传方法和相互作用者的筛选，我们已经发现了一个三组件模块。该模块的第一个组成部分是毛茸茸的激酶BIN 2，它介导信号整合。第二个组成部分是保守的TRAPPII多亚基拴系复合物，介导的决策过程中的trans-Golgi网络（TGN）。第三个组件包括一个Rab GTP酶家族，它们被设置为在前两个组件的下游执行决策。为了剖析BIN 2-TRAPP-RAB模块信号会聚的分子机制，我们将结合蛋白质组学、生物化学、遗传学和高分辨率共聚焦显微镜技术。我们的具体目标是（1）阐明油菜素类固醇（BR）诱导的翻译后修饰对TGN相关TRAPP II系链复合物的组装和功能的影响，（2）表征TRAPP复合物和Rab GTP酶之间的功能相互作用，以及（3）评估这些如何指导分选和运输决策。我们的研究将确定TGN信号调节有助于细胞分裂，伸长，生长各向异性和分生组织功能的分选决定的分子机制。这里获得的机制见解将为理解植物适应性生长和分配决策奠定基础。