Novel functions of nuclear transport factors : stress-response mechanism of cell nucleus

核转运因子的新功能：细胞核的应激反应机制

• 批准号: 21247032
• 负责人: YONEDA Yoshihiro
• 金额: $ 29.29万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21247032/
• 关键词: 核輸送; ストレス応答; importinα; 細胞核; 遺伝子発現; 細胞死; importin α; マイクロアレイ

Various cellular stresses induce the nuclear accumulation of importinα. However, it is unknown whether accumulated importinαperforms roles in the nucleus. In this study, we found that nuclear-retained importinα2binds with DNase I-sensitive nuclear component(s) and exhibits selective up-regulation of mRNA encoding Serine/ threonine kinase35(STK35). Chromatin immunoprecipitation and promoter analysis demonstrated that importinα2binds to the promoter region of STK35and accelerates its transcription. Furthermore, constitutive overexpression of STK35proteins enhances caspase-independent cell death under oxidative stress conditions. These results reveal that nuclear-localized importinα2influences gene expression and contributes directly to cell fate outcomes including non-apoptotic cell death. This is the first evidence showing that importinαhas novel functions other than nuclear protein import in the nucleus.

各种细胞应力诱导进口量的核加速度。但是，尚不清楚累积的Importin是否在核中起角色。在这项研究中，我们发现具有DNase I敏感核成分的核保留的核进口量，并显示出编码丝氨酸/苏氨酸基因酶35（STK35）的mRNA的选择性上调。染色质免疫沉淀和启动子分析表明，进口素α2BIND与STK35的启动子区域并加速其转录。此外，在氧化物应激条件下，STK35蛋白质的本构过表达增强了与胱天蛋白酶无关的细胞死亡。这些结果表明，核局部化导入基因表达，直接促进包括非凋亡细胞死亡在内的细胞脂肪结果。这是第一个证据表明，进口的核蛋白在核蛋白中的作用。

项目成果

Effective culture conditions for the induction of pluripotent stem cells

• DOI: 10.1016/j.bbagen.2010.04.004
• 发表时间: 2010-09-01
• 期刊: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
• 影响因子: 3
• 作者: Okada, Minoru;Oka, Masahiro;Yoneda, Yoshihiro
• 通讯作者: Yoneda, Yoshihiro

Cytoplasmic polyadenylation element-like sequences are involved in dendritic targeting of BDNF mRNA in hippocampal neurons

• DOI: 10.1016/j.febslet.2010.06.040
• 发表时间: 2010-08-04
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Oe, Souichi;Yoneda, Yoshihiro
• 通讯作者: Yoneda, Yoshihiro

Differential nuclear import is a novel regulatory mechanism for maintaining pluripotency of ES cells

差异核输入是维持 ES 细胞多能性的新型调控机制

• 发表时间: 2010
• 作者: Yasuhara;N.;Yamagishi;R.;Arai;Y.;Yanagida;T;Kaneko;H. and Yoneda;Y.
• 通讯作者: Y.

Importin alpha mediates biased translocation through nuclear pores in conjunction with Nup153

Importin alpha 与 Nup153 一起介导通过核孔的偏向易位

• 发表时间: 2011
• 作者: Ogawa;Y.;Miyamoto;Y.;Oka;M. and Yoneda;Y
• 通讯作者: Y

The modulation in glycosylation state of nucleoporins in cell differentiation

细胞分化中核孔蛋白糖基化状态的调节

• 发表时间: 2010
• 作者: Mizuguchi C;Oka M;Moriyama T;Tachibana T;Yoneda Y.;Chiaki Mizuguchi
• 通讯作者: Chiaki Mizuguchi