Mechanism and function of endocytosis in sucrose accumulation of plant cells

内吞作用在动物和植物中具有广泛的功能。网格蛋白包被囊泡（CCV）的形成是内吞作用的第一步，在动物细胞中主要是由动力蛋白完成的。然而，其在植物细胞中的分子机制尚不清楚。为了鉴定参与植物细胞内吞CCV形成的动力蛋白（dynamin-related protein, DRPs），我们比较了两种结构不同的拟南芥DRP2B和DRP1A的行为。在拟南芥细胞中，DRP2B和DRP1A在质膜上共定位并一起组装/拆卸。尽管DRP2B和DRP1A分子性质不同，但它们共同参与拟南芥细胞内吞CCV的形成。

Endocytosis performs a wide range of functions in animals and plants. Clathrin-coated vesicle (CCV) formation is an initial step of endocytosis, and in animal cells is largely achieved by dynamins. However, little is known of its molecular mechanisms in plant cells. To identify dynamin-related proteins (DRPs) involved in endocytic CCV formation in plant cells, we compared the behaviors of two structurally different Arabidopsis DRPs, DRP2B and DRP1A. DRP2B and DRP1A colocalized and assembled/disassembled together at the plasma membrane in Arabidopsis cells. DRP2B and DRP1A participate together in endocytic CCV formation in Arabidopsis cells despite the difference of their molecular properties.