Mechanism and function of endocytosis in sucrose accumulation of plant cells

内吞作用在动植物中发挥着广泛的功能。笼蛋白包裹的囊泡(CCV)的形成是内吞作用的第一步，在动物细胞中主要是通过动力素来实现的。然而，对其在植物细胞中的分子机制知之甚少。为了确定参与植物细胞内质CCV形成的动力蛋白相关蛋白(DRPs)，我们比较了两个结构不同的拟南芥DRP2B和DRP1A的行为。在拟南芥细胞中，DRP2B和DRP1A在质膜上共定位和组装/分解在一起。尽管DRP2B和DRP1a的分子特性不同，但它们共同参与了拟南芥细胞内CCV的形成。

Endocytosis performs a wide range of functions in animals and plants. Clathrin-coated vesicle (CCV) formation is an initial step of endocytosis, and in animal cells is largely achieved by dynamins. However, little is known of its molecular mechanisms in plant cells. To identify dynamin-related proteins (DRPs) involved in endocytic CCV formation in plant cells, we compared the behaviors of two structurally different Arabidopsis DRPs, DRP2B and DRP1A. DRP2B and DRP1A colocalized and assembled/disassembled together at the plasma membrane in Arabidopsis cells. DRP2B and DRP1A participate together in endocytic CCV formation in Arabidopsis cells despite the difference of their molecular properties.