Nuclear encoded chloroplast ribosomal protein genes ; genomic structure and organ-specific gene expression.
核编码叶绿体核糖体蛋白基因;
基本信息
- 批准号:08660004
- 负责人:
- 金额:$ 1.66万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
A cDNA library was constructed with poly(A)^+RNA prepared from 10-day-old rice seedlings. I have isolated three nuclear genes, rpl9, rpl12-1, rpl12-2, rpl13, rpl15, rp121, rpl24, rpl28, rps1 and rps9, that encode chloroplast ribosomal proteins L9, L12, L13, L15, L21, L24, L28, S1 and S9 respectively. Using these clones as probes, these nuclear encoded genes were located on molecular linkage map of rice. Seven loci were located on 5 differnt chromosomes of rice. They were not clustered but dispersed throughout the rice genome.Expression analysis of rpl12-1, rpl12-2, rpl13, rpl24 by Northern blotting reveals that these genes were expessed in leaves of 10-day-old rice seedlings regardless of the presence of light, but not in roots. These data suggest that nuclear encoded ribosomal protein genes were expressed in an organ specific manner.The genomic clones of rpl13 and rpl24 were isolated, and transcription start sites were determined by S1-nulcease mapping and primer extension. It is suggested that both genes have multiple sites of transcription initiation, and that each site was employed for transcription initiation differently in specific organ.
以10天龄水稻幼苗为材料,用Poly(A)^+RNA构建了一个cDNA文库。我已分离到三个核基因rpl9、rpl12-1、rpl12-2、rpl13、rpl15、rp121、rpl24、rpl28、rps 1和rps 9,它们分别编码叶绿体核糖体蛋白L9、L12、L13、L15、L21、L24、L28、S1和S9。以这些克隆为探针,将这些核编码基因定位在水稻分子连锁图谱上。7个基因座位于水稻的5条不同染色体上。通过Northern blotting对rpl12-1、rpl12-2、rpl13、rpl24基因的表达分析表明,这些基因在10日龄水稻幼苗叶片中表达,而在根中不表达。这些数据表明,核糖体蛋白基因以器官特异的方式表达。分离了RPL13和RPL24的基因组克隆,并通过S1-nulce定位和引物延伸确定了转录起始点。推测这两个基因都有多个转录起始点,而且在特定器官中每个起始点的转录起始点是不同的。
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Itoh Y.: "Transcript levels of tendem-arranged alternative oxidase genes in rice are increased by low temperature." Gene. 203. 121-129 (1997)
Itoh Y.:“低温会增加水稻中十串排列的替代氧化酶基因的转录水平。”
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
Nobuhiro Tsutsunm: "Molecular cloning and nucleotide sequencing of nuclear genes coding for the chloreplast ribosumal proteins L13,L24,L28 of rice (Opse salival)" Plant Science. 121. 167-174 (1996)
Nobuhiro Tsutsunm:“编码水稻(Opse 唾液)叶绿体核糖蛋白 L13、L24、L28 的核基因的分子克隆和核苷酸测序”《植物科学》。
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- 影响因子:0
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"Characterization of two rice genes for nuclearencoded chloroplast ribosomal protein L12 and phylogenetic analysis of the acquisition of transit peptides and gene duplication." Theor. Appl. Genet. ( in press ).
“核编码叶绿体核糖体蛋白 L12 的两个水稻基因的表征以及转运肽获取和基因复制的系统发育分析。”
- DOI:
- 发表时间:
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- 影响因子:0
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日下 宗規: "Characterization of two rice genes for nuclear-encoded chloroplast ribosomal protein L12 and phylogenetic analysis of the acquisition of transit peptides and gene duplication." Theor.Appl.Genet.(印刷中).
Munenori Kusaka:“核编码叶绿体核糖体蛋白 L12 的两个水稻基因的表征以及转运肽和基因复制获取的系统发育分析。”(正在出版)。
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- 影响因子:0
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金澤 章: "Phylogenetic relationships in the genus Nelumbo based on polymorphism and quantitative variations in mitochondrial DNA." Genes Genet.Syst.(印刷中).
Akira Kanazawa:“基于线粒体 DNA 的多态性和定量变异的莲属系统发育关系”(Genes Genet.Syst)。
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- 影响因子:0
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TSUTSUMI Nobuhiro其他文献
TSUTSUMI Nobuhiro的其他文献
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{{ truncateString('TSUTSUMI Nobuhiro', 18)}}的其他基金
Mechanism and function of endocytosis in sucrose accumulation of plant cells
植物细胞蔗糖积累中内吞作用的机制和功能
- 批准号:
21248002 - 财政年份:2009
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
The role of mitochondria in programmed cell death in higher plants
线粒体在高等植物程序性细胞死亡中的作用
- 批准号:
18208002 - 财政年份:2005
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
The molecular mechanism of mitochondrial fission and fusion in higher plants.
高等植物线粒体裂变与融合的分子机制。
- 批准号:
15208001 - 财政年份:2003
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
The possibility of nitrogen fixation by genetic improving the rice chioroplast
通过基因改良水稻叶绿体固氮的可能性
- 批准号:
12460001 - 财政年份:2000
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Expression of nuclear genes for chloroplast ribosomal proteins.
叶绿体核糖体蛋白核基因的表达。
- 批准号:
10660006 - 财政年份:1998
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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