Studies of pathological involvement of Sox9 in osteoarthritis and discovery of new treatment for osteoarthritis

Sox9与骨关节炎的病理参与研究及骨关节炎新疗法的发现

• 批准号: 21249078
• 负责人: AKIYAMA Haruhiko
• 金额: $ 30.53万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21249078/
• 关键词: 変形性関節症; Sox9; 遺伝子改変マウス

Overexpression of Sox9 in articular chondrocytes resulted in delay of osteoarthritis change. In addition, genome-wide screening using GeneChip with chondrocytes derived mouse articular cartialge, Sox9-EGFP mice, and Sox9-EGFP null chimera identified one gene involved in osteoarthritis and one gene induced by Sox9. Furthermore, screening of about 10000 chemical reagents using Sox9-EGFP chondrocytes revealed 7 chemical reagents which has Sox9-indecing activity.

Sox 9在关节软骨细胞中的过表达导致骨关节炎改变的延迟。此外，使用基因芯片对软骨细胞来源的小鼠关节软骨、Sox 9-EGFP小鼠和Sox 9-EGFP无效嵌合体进行全基因组筛选，鉴定出一个参与骨关节炎的基因和一个由Sox 9诱导的基因。此外，使用Sox 9-EGFP软骨细胞筛选约10000种化学试剂，发现7种化学试剂具有Sox 9-indecing活性。

项目成果

軟骨における転写制御機構から病態解析・治療へ

从软骨转录控制机制到病理分析和治疗

• 发表时间: 2010
• 作者: Sugawara T.;Yoshida S.;Wada K.;Hirose S.;Iwasa H.;Kaneko S.;Terashima K;秋山治彦
• 通讯作者: 秋山治彦

Generation of transgenic mice for conditional overexpression of Sox9.

• DOI: 10.1007/s00774-010-0206-z
• 发表时间: 2011-01
• 期刊: JOURNAL OF BONE AND MINERAL METABOLISM
• 影响因子: 3.3
• 作者: Kim, Youngwoo;Murao, Hiroki;Yamamoto, Koji;Deng, Jian Min;Behringer, Richard R.;Nakamura, Takashi;Akiyama, Haruhiko
• 通讯作者: Akiyama, Haruhiko

Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and mediator subunit 25.

• DOI: 10.1038/ncomms1242
• 发表时间: 2011
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Nakamura, Yukio;Yamamoto, Koji;He, Xinjun;Otsuki, Bungo;Kim, Youngwoo;Murao, Hiroki;Soeda, Tsunemitsu;Tsumaki, Noriyuki;Deng, Jian Min;Zhang, Zhaoping;Behringer, Richard R.;de Crombrugghe, Benoit;Postlethwait, John H.;Warman, Matthew L.;Nakamura, Takashi;Akiyama, Haruhiko
• 通讯作者: Akiyama, Haruhiko

Continuous cell supply from a Sox9-expressing progenitor zone in adult liver, exocrine pancreas and intestine

• DOI: 10.1038/ng.722
• 发表时间: 2011-01-01
• 期刊: NATURE GENETICS
• 影响因子: 30.8
• 作者: Furuyama, Kenichiro;Kawaguchi, Yoshiya;Uemoto, Shinji
• 通讯作者: Uemoto, Shinji

Sox9-expressing Prectusors are the Cellular Origin of the Cruciate Ligament of the Knee Joint and the Limb Tendons

表达 Sox9 的前肌是膝关节十字韧带和肢体肌腱的细胞起源

• 发表时间: 2010
• 期刊: Genesis
• 影响因子: 1.5
• 作者: Tsunemitsu Soeda;Jian Min Deng;Haruhiko Akiyama.;et al.
• 通讯作者: et al.