A study of bone atrophy treatment by detection of factors in osteocytes involved in mechanical stress
A study of bone atrophy treatment by detection of factors in osteocytes involved in mechanical stress
批准号:
23659717
负责人:
AKIYAMA Haruhiko
金额:
$2.33万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
中文摘要
使用废骨骨细胞进行全基因组筛选显示BK通道基因上调。RT-PCR结果显示,骨细胞表达KCNMB1、2和4亚基基因。构建CaK-GFP转基因小鼠,再构建Col1a1-mRFP1;制备了CaK-GFP双半转基因小鼠。当使用BK通道开启剂或阻滞剂时,我们正在尝试使用共聚焦显微镜对这些小鼠的成骨细胞和成骨细胞进行实时成像。
英文摘要
Genome-wide screening using osteocytes in disused bone showed upregulation of BK channel genes. RT-PCR revealed that osteocytes express KCNMB1, 2 and 4 subunit genes. CaK-GFP transgenic mice were generated, and then Col1a1-mRFP1; CaK-GFP double hemi-transgenic mice were produced. We are trying live imaging of osteoblasts and osteclasts in these mice using confocal microscope when BK channel opener or blocker reagents are administered.
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会议论文
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依托单位:
海外基金