Functional analysis of the molecular markers of the growth cone

生长锥分子标记的功能分析

• 批准号: 22240040
• 负责人: IGARASHI MICHIHIRO
• 金额: $ 31.87万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22240040/
• 关键词: 成長円錐; 神経細胞極性; コンドロイチン硫酸; ヘパラン硫酸; M6a; シンタキシン; 軸索再生; 極性形成機構; 小胞輸送; アクチン繊維; ノックアウトマウス; 行動解析; GAP-43; 神経極性形成; 遺伝子改変マウス

We produced the CSGalNAcT1 (T1)-KO mice for anlyzing the in vivo growth cone functions. By producing the spinal cord injury (SCI) model, we observed that in T1-KO, markerd axon regrowth occurred. In these mice, nor only chondroitin sulfate (CS), an inhibitor of the growth cone, was decreased, but also heparan sulfate (HS), one of its stimulators, also was up-regulated. We concluded that CS/HS balance is very important for the effective axon regeneration. We also obtained the evidence that GPM6a is invovled in the determination of the neuronal polarity.

我们制备了CSGalNAcT 1（T1）-KO小鼠用于分析体内生长锥功能。通过建立脊髓损伤（SCI）模型，我们观察到在T1-KO中，发生了显著的轴突再生。在这些小鼠中，不仅硫酸软骨素（CS），生长锥的抑制剂，减少，而且硫酸乙酰肝素（HS），其刺激剂之一，也上调。结论：CS/HS平衡对轴突再生的有效性至关重要。我们还获得了GPM 6a参与神经元极性决定的证据。

项目成果

Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development.

• DOI: 10.1042/bj20100847
• 发表时间: 2010-11-15
• 期刊: The Biochemical journal
• 作者: Watanabe Y;Takeuchi K;Higa Onaga S;Sato M;Tsujita M;Abe M;Natsume R;Li M;Furuichi T;Saeki M;Izumikawa T;Hasegawa A;Yokoyama M;Ikegawa S;Sakimura K;Amizuka N;Kitagawa H;Igarashi M
• 通讯作者: Igarashi M

Mice lacking collapsin response mediator protein 1 manifest hyperactivity, impaired learning and memory, and impaired prepulse inhibition.

• DOI: 10.3389/fnbeh.2013.00216
• 发表时间: 2013
• 期刊: Frontiers in behavioral neuroscience
• 影响因子: 3
• 作者: Yamashita N;Takahashi A;Takao K;Yamamoto T;Kolattukudy P;Miyakawa T;Goshima Y
• 通讯作者: Goshima Y

Absence of BRINP1 in mice causes increase of hippocampal neurogenesis and behavioral alterations relevant to human psychiatric disorders.

• DOI: 10.1186/1756-6606-7-12
• 发表时间: 2014-02-14
• 期刊: Molecular brain
• 影响因子: 3.6
• 作者: Kobayashi M;Nakatani T;Koda T;Matsumoto K;Ozaki R;Mochida N;Takao K;Miyakawa T;Matsuoka I
• 通讯作者: Matsuoka I

Chondroitin sulphate N-acetylgalactosaminyl-transferase-1 inhibits recovery from neural injury.

• DOI: 10.1038/ncomms3740
• 发表时间: 2013
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Takeuchi, Kosei;Yoshioka, Nozomu;Onaga, Susumu Higa;Watanabe, Yumi;Miyata, Shinji;Wada, Yoshino;Kudo, Chika;Okada, Masayasu;Ohko, Kentaro;Oda, Kanako;Sato, Toshiya;Yokoyama, Minesuke;Matsushita, Natsuki;Nakamura, Masaya;Okano, Hideyuki;Sakimura, Kenji;Kawano, Hitoshi;Kitagawa, Hiroshi;Igarashi, Michihiro
• 通讯作者: Igarashi, Michihiro

PRICKLE1 interaction with SYNAPSIN I reveals a role in autism spectrum disorders.

• DOI: 10.1371/journal.pone.0080737
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Paemka L;Mahajan VB;Skeie JM;Sowers LP;Ehaideb SN;Gonzalez-Alegre P;Sasaoka T;Tao H;Miyagi A;Ueno N;Takao K;Miyakawa T;Wu S;Darbro BW;Ferguson PJ;Pieper AA;Britt JK;Wemmie JA;Rudd DS;Wassink T;El-Shanti H;Mefford HC;Carvill GL;Manak JR;Bassuk AG
• 通讯作者: Bassuk AG