Generating molecular diversity by the wide range of polyketide synthases

通过多种聚酮合酶产生分子多样性

• 批准号: 22241054
• 负责人: NOGUCHI Hiroshi
• 金额: $ 26.62万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22241054/
• 关键词: ゲノム; 酵素工学; 生理活性; biosynthesis; 生合成; メロテルペノイド; 分子多様性; テルペン環化酵素; 酵素; テレトニン; プレニル基転移酵素; バイオテクノロジー

Novel type III PKS was isolated from Garcinia mangostana L. , which contains mainly prenylated xanthones. A key prenyltransferase involved in the biosynthesis of abundant fungal meroterpenoids derived from 3,5-dimethylorsellinic was identified. The prenylated product serves as a key intermediate in the biosynthesis of series of meroterpenoids in fungi. With Aspergillus nidlans, Metabolic engineering of yeast for heterologous production of tryprostatins and other compounds was developed through our engineered strain of Saccharomyces cerevisiae, SCKW5. Further trial to architect comprehensive system to form secondary metabolites with Chaetomium globosum is under progress.

新型III型PKS是从Garcinia mangostana L.中分离出来的，其主要含有异戊二烯化的呫吨酮。鉴定出一种关键的异戊烯基转移酶，该酶参与源自 3,5-二甲基阿赖烯的丰富真菌类萜的生物合成。异戊二烯化产物是真菌中一系列类萜生物合成的关键中间体。利用构巢曲霉，通过我们的酿酒酵母工程菌株 SCKW5 开发了用于异源生产胰前列腺素和其他化合物的酵母代谢工程。利用球毛壳菌形成次级代谢产物的综合系统的进一步试验正在进行中。

项目成果

Establishing a new methodology for genome mining and biosynthesis of natural products through fungal molecular genomics

• DOI: 10.1055/s-0032-1320342
• 发表时间: 2012-07
• 期刊: Planta Medica
• 影响因子: 2.7
• 作者: H. Noguchi

A novel triterpene synthase gene from a monocot plant, Imperata cylindrical

来自单子叶植物白茅的新型三萜合酶基因

• 发表时间: 2012
• 作者: 池内佑一郎;喜多隆;他;荒井 雅吉;Tetsuo Kushiro
• 通讯作者: Tetsuo Kushiro

A chemical library by engineered plant polyketide synthase

工程化植物聚酮合酶的化学文库

• 发表时间: 2010
• 作者: Y.Shoji;T.Morioka;Y.Okada;H. Noguchi
• 通讯作者: H. Noguchi

Functional reconstitution of the saframycin biosynthetic enzymes

红霉素生物合成酶的功能重建

• 发表时间: 2012
• 作者: Kawakami;K.;H. Noguchi;安岡宏和;森浩禎;T. Kita;可知直毅;K. Watanabe
• 通讯作者: K. Watanabe

Benzophenone synthase from Garcinia mangostana

山竹藤黄二苯甲酮合酶

• 发表时间: 2012
• 期刊: L. pericarps. Phytochemistry
• 作者: N. Nualkaew;H. Morita;Y. Shimokawa;K. Kinjo;T. Kushiro;W. De-Eknamkul;Y. Ebizuka;I. Abe
• 通讯作者: I. Abe