Methanogenesis under high pressure conditions mimicking subsurface environments

我们调查了生物产甲烷是否在高压条件下发生(模拟地下环境)。我们使用了纯培养的水遗传营养和醋解甲烷菌，以及一种乙酸酯氧化产氢合生菌，以及来自油田的环境地下样本。1)在高压条件下(高达15 Mpa)，成功地进行了产氢产甲烷菌和裂解产甲烷菌的纯培养以及共营养共培养。请注意，合养共培养在如此高压的条件下生长并产生甲烷。2)以甲氧基苯甲酸酯为模型干酪根衍生物，丰富了能够将其降解为甲烷的群落。3)在利用醋酸盐的产甲烷群落中，CO2浓度是驱动乙酸乙酯氧化或合养乙酸酯氧化以及随后H2和CO2转化为甲烷的关键因素。

We investigated whether biological methanogensis occurs under high pressure conditions (mimicking subsurface environments). We used pure cultures of hydrogenotrophic and aceticlastic methanogens, and an acetate oxidizing hydrogen producing syntroph as well as environmental subsurface samples taken from an oil field. We obtained the following results: 1) pure cultures of hydrogenotrophic and aceticlastic methanogens, and syntrophic coculture were successfully cultivated under high pressure conditions (up to 15MPa). Note that the syntrophic coculture grew and produced methane under such high pressure conditions. 2) Methoxylated benzoates were used as model kerogen derivatives to enrich communities that were capable of degrading them to produce methane. 3) In the acetate-utilizing methane-producing community, CO2 concentration is the crucial factor driving either aceticlastic methanogenesis or syntrophic acetate oxidation and subsequent methanogenesis from H2 and CO2.