Analysis of Muscle and Tendon Development and its application to Medicine

肌肉和肌腱发育分析及其医学应用

• 批准号: 23249071
• 负责人: ASAHARA Hiroshi
• 金额: $ 30.7万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23249071/
• 关键词: 筋; 腱; Mkx; RP58; Mｋｘ; 関節; 靭帯; ラブドミオザルコーマ

The research focused on the formation of muscle-tendon structure complex, which is essential for motor function. RP58, a transcription factor required in the development of muscle and Mkx, essential in normal growth of tendons and ligaments were investigated. In order for to determine the mechanism for Mkx expression, promoter/enhancer region of Mkx was localized and 3 candidate transcription factors were identified. Mkx regulated genes were also investigated and identified. RP58 is known to bind to DNMT3, a de novo DNA methyltransferase, and hence DNA methylation was analyzed genome-wide and regions of methylation activity were identified.

研究的重点是肌肉-肌腱复合体的形成，这是运动功能所必需的。对肌肉发育所需的转录因子RP58和肌腱韧带正常生长所必需的MKX进行了研究。为了确定MKX的表达机制，我们对MKX的启动子/增强子区进行了定位，并鉴定了3个候选转录因子。并对MKX调控基因进行了研究和鉴定。RP58已知与从头DNA甲基转移酶DNMT3结合，因此对DNA甲基化进行了全基因组分析，并确定了甲基化活性区域。

项目成果

Identification of novel transcription-regulating genes expressed during murine molar development

• DOI: 10.1002/dvdy.23808
• 发表时间: 2012-07-01
• 期刊: DEVELOPMENTAL DYNAMICS
• 影响因子: 2.5
• 作者: Uchibe, Kenta;Shimizu, Hirohito;Asahara, Hiroshi
• 通讯作者: Asahara, Hiroshi

FOXL2 transcriptionally represses Sf1 expression by antagonizing WT1 during ovarian development in mice

• DOI: 10.1096/fj.13-246108
• 发表时间: 2014-05-01
• 期刊: FASEB JOURNAL
• 影响因子: 4.8
• 作者: Takasawa, Kei;Kashimada, Kenichi;Koopman, Peter
• 通讯作者: Koopman, Peter

MAML1 enhances the transcriptional activity of Runx2 and plays a role in bone development.

• DOI: 10.1371/journal.pgen.1003132
• 发表时间: 2013
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Watanabe T;Oyama T;Asada M;Harada D;Ito Y;Inagawa M;Suzuki Y;Sugano S;Katsube K;Karsenty G;Komori T;Kitagawa M;Asahara H
• 通讯作者: Asahara H