Biomedical Application of Gas Biology through Multidisciplinary Approaches

通过多学科方法进行气体生物学的生物医学应用

• 批准号: 17GS0419
• 负责人: SUEMATSU Makoto
• 金额: $ 211.91万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Creative Scientific Research
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17GS0419/
• 关键词: 一酸化炭素; ガスバイオロジー; 酸素センサー; メタボローム; cytochrome oxidase; 構造生物学; ミトコンドリア; Hypoxia inducible factor; 生体情報工学; グルタチオン; 一酸化窒素

The multiplicity of gas actions and gas targets and the difficulty in measuring local gas concentrations obscures detailed mechanisms whereby gases exert their actions, and many questions remain unanswered. In this project, advanced mass spectrometry combined with high-performance computer technology allowed us to mine biological mechanisms for gas-responsive signal transducing systems. We also take an integrated approach to the interaction of gases by considering the physiological significance of CO, NO, and H2S on metabolic systems and energy-supporting enzyme systems including cytochrome c oxidase, a key target and central mediator of mitochondrial respiration. By evaluating gas-mediated control functions from both in vitro and in vivo perspectives, we attempted to elaborate on the complex multiple interactions of O(2), NO, CO, and H2S

气体作用和气体目标的多样性以及测量局部气体浓度的困难掩盖了气体发挥作用的详细机制，许多问题仍然没有答案。在这个项目中，先进的质谱与高性能计算机技术相结合，使我们能够挖掘气体响应信号转导系统的生物机制。我们还采取了综合的方法来考虑CO，NO和H2S的代谢系统和能量支持酶系统，包括细胞色素c氧化酶，线粒体呼吸的关键目标和中央调解人的生理意义的气体的相互作用。通过从体外和体内两个角度评估气体介导的控制功能，我们试图阐述O（2）、NO、CO和H2S的复杂的多重相互作用

项目成果

The effects of cilostazol on tissue oxygenation upon an ischemic-reperfusion injury in the mouse cerebrum.

西洛他唑对小鼠大脑缺血再灌注损伤时组织氧合的影响。

• 发表时间: 2008
• 作者: Morikawa T.;Hattori K.;Kajimura M.;Suematsu M.
• 通讯作者: Suematsu M.

Multiple high-throughput analyses monitor the response of E-coli to perturbations

• DOI: 10.1126/science.1132067
• 发表时间: 2007-04-27
• 期刊: SCIENCE
• 影响因子: 56.9
• 作者: Ishii, Nobuyoshi;Nakahigashi, Kenji;Tomita, Masaru
• 通讯作者: Tomita, Masaru

Metabolic Remodeling Induced by Mitochondrial Aldehyde Stress Stimulates Tolerance to Oxidative Stress in the Heart

• DOI: 10.1161/circresaha.109.206607
• 发表时间: 2009-11-20
• 期刊: CIRCULATION RESEARCH
• 影响因子: 20.1
• 作者: Endo, Jin;Sano, Motoaki;Fukuda, Keiichi
• 通讯作者: Fukuda, Keiichi

Tissue- and context-dependent modulation of hormonal sensitivity of glucocorticoid-sensitive genes by HEXIM1

HEXIM1 对糖皮质激素敏感基因的激素敏感性进行组织和环境依赖性调节

• 发表时间: 2008
• 期刊: Mol. Endocrinol. 22
• 作者: Shimizu;N.;Yoshikawa;N. Wada;T.;Handa;H.;Sano;M.;Fukuda;K.;Suematsu;M.;Sawai;T.;Morimoto;C.;Tanaka;H.
• 通讯作者: H.

α-ketoglutarate oxidoreductase, an essential salvage enzyme of energy metabolism, in coccoid form of Helicobacter pylori.

α-酮戊二酸氧化还原酶，一种能量代谢的重要挽救酶，以幽门螺杆菌的球状形式存在。

• 发表时间: 2008
• 期刊: Biochem. Biophys. Res. Commun. 376
• 作者: Tsugawa;H.;Suzuki;H.;Nakagawa;I.;Nishizawa;T.;Saito;Y.;Suematsu. M.;Hibi;T.
• 通讯作者: T.