Comparing osmotic stress responses between wild barley cultivar, landrace and domesticated barley: chemical, physiological and molecular adaptations of apoplastic barriers in leaves and roots

比较野生大麦品种、地方品种和驯化大麦之间的渗透胁迫反应：叶和根质外体屏障的化学、生理和分子适应

• 批准号: 511193270
• 负责人: Professor Dr. Lukas Schreiber
• 金额: --
• 依托单位: Institute of Plant Sciences
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/511193270?language=en
• 关键词: Comparing; osmotic; stress; responses; between

In this project we will investigate the adaptations of apoplastic barriers in leaves (epidermal cutin and wax deposition) and roots (endodermal suberin deposition) to osmotic stress comparing wild barley, landraces and domesticated barley. This approach includes (i) the microscopic and chemical-analytical investigation of apoplastic barriers over the length of barley leaves and roots, (ii) the investigation of the gene expression (transcriptomics) over the length of barley leaves and roots, (iii) the metabolomic and ionomic investigation of barley leaves and roots in order to compare apoplastic cell wall responses with symplastic cellular responses and finally (iv) the quantitative analysis of water transport across barley leaf and root apoplastic barriers. By comparing wild barley, landraces and domesticated barley, deeper insights in the adaptation of barley leaf and root apoplastic barriers to osmotic stress can be obtained. This knowledge could help in future in barley breeding to achieve more stress tolerant cultivars.

在这个项目中，我们将调查的适应性质外体障碍叶（表皮角质和蜡沉积）和根（内胚层木栓质沉积）渗透胁迫比较野生大麦，地方品种和驯化大麦。该方法包括（i）大麦叶和根长度上质外体屏障的显微镜和化学分析研究，（ii）基因表达的研究，（转录组学）在大麦叶和根的长度上，（iii）大麦叶和根的代谢组学和离子组学研究，以比较质外体细胞壁反应与共质体细胞反应，最后（iv）大麦叶和根质外体屏障水分运输的定量分析。通过比较野生大麦、地方品种和驯化大麦，可以更深入地了解大麦叶和根质外体屏障对渗透胁迫的适应。这些知识可以帮助在未来的大麦育种，以获得更多的耐胁迫品种。