Structur and function of mutants of the 20S proteasome from S. cerevisiae (Struktur und Funktion von Mutanten des 20S Proteasoms aus S. cerevisiae)

酿酒酵母 20S 蛋白酶体突变体的结构和功能

• 批准号: 5112948
• 负责人: Professor Dr. Robert Huber
• 金额: --
• 依托单位: Emeritus-Gruppe für Strukturforschung
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 1998
• 资助国家: 德国
• 起止时间: 1997-12-31 至 2002-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5112948?language=en
• 关键词: Structur; function; mutants; 20S; proteasome

Based on the crystal structure of the 20S proteasome from yeast, determined in 1997, we want to investigate structure and function of mutants and peptide complexes, with special emphasis on those, that influence substrate access to the proteolytic chamber. We would like to determine the role of proteasome associated proteins looking at the structure of either isolated components or protein complexes. In particular, we want to focus on the ATPase subunits of the yeast and archaebacterial proteasomes. The eubacterial proteasome analogue Hs1VU will also be analysed structurally.

基于1997年确定的酵母20S蛋白酶体的晶体结构，我们要研究突变体和肽复合物的结构和功能，特别强调那些影响底物进入蛋白水解室的结构和功能。我们想确定蛋白酶体相关蛋白质的作用，无论是孤立的成分或蛋白质复合物的结构。特别是，我们想集中在酵母和古细菌蛋白酶体的ATP酶亚基。还将对真细菌蛋白酶体类似物Hs1VU进行结构分析。