Animal models and fungal mutants

摘要 模式生物体核心的目标是执行所提议的生物危害研究项目 在项目1-4和核心本身内，并保持这两个不同菌株的培养集合 球孢子虫属的种类。这个核心有两个目标。一是开展所提出的所有活动和实验 在涉及活的、剧毒的球孢子虫物种的项目中，球孢子虫和波萨迪亚球孢球虫， 因为它们被归类为BSL3病原体。核心C的另一个目标是利用新方法来有针对性地 两种球虫保守基因的突变，并测试突变体的生长能力 媒介和小鼠体内。BSL3设施将能够在小鼠身上测试项目1、2和3的新假设，并 从目标1中提出的小鼠实验结果中产生关于免疫反应的新假设 向项目3提供信息。目标2中的拟议研究与项目4直接相关，并将揭示 有助于毒力的真菌基因，这可能是治疗的新靶点，并识别宿主基因 和控制对球虫感染的免疫反应的反应途径，这可能揭示新的 生物制剂的治疗靶点。

Abstract The goals of the Model Organisms Core are to carry out the biohazardous research projects that are proposed in the Projects 1-4 and within the Core itself, and to maintain the culture collection of different strains of both species of Coccidioides. This Core has two aims. One is to carry out all the activities and experiments proposed in the projects that involve live, virulent Coccidioides species, Coccidioides immitis and Coccidioides posadasii, as they are classified as BSL3 pathogens. The other aim of the Core C is to utilize new methods to make targeted mutations in conserved genes in both species of Coccidioides, and to test the mutants for their ability to grow on media and in mice. The BSL3 facility will enable testing new hypotheses from Projects 1, 2, & 3 in mice, and to generate new hypotheses about immune responses from the results of mouse experiments proposed in Aim 1 to inform Project 3. The proposed studies in Aim 2 are directly related to Project 4 and will also shed light on the fungal genes that contribute to virulence, which may be new targets for therapeutics, and to identify host genes and response pathways that control immune responses to Coccidioides infection, which may reveal new therapeutic targets for biological agents.