Animal models and fungal mutants

动物模型和真菌突变体

• 批准号: 10356726
• 负责人: Joshua Fierer
• 金额: $ 28.7万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-24 至 2026-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10356726
• 关键词: Affect; Animal Model; Biological Assay; Biological Metamorphosis; Biological Products; CRISPR/Cas technology; Cells; Chitinase; Chronic; Clinical Microbiology; Coccidioides; Coccidioides immitis; Coccidioides posadasii; Coccidioidomycosis; Collection; Complex; Dangerousness; Disease Outbreaks; Environment; Female; Fungal Genes; Gene Mutation; Genes; Goals; Immune response; Immunogenetics; In Vitro; Inbred Strains Mice; Inbreeding; Infection; Laboratories; Laboratory Personnel; Life Cycle Stages; Light; Lung infections; Mediating; Methods; Modeling; Molds; Mus; Mutation; Mycelium; Pathogenesis; Pathway interactions; Plasmids; Play; Predisposition; Recombinants; Reproduction spores; Research; Research Activity; Research Personnel; Research Project Grants; Resistance; Role; Soil; Structure; System; Technology; Testing; Tissues; Virulence; Virulent; Woman; asexual; base; biosafety level 3 facility; culture plates; experience; experimental study; fungus; gene function; infection risk; men; mouse model; mutant; new therapeutic target; pathogen; research facility; response; tool

Abstract The goals of the Model Organisms Core are to carry out the biohazardous research projects that are proposed in the Projects 1-4 and within the Core itself, and to maintain the culture collection of different strains of both species of Coccidioides. This Core has two aims. One is to carry out all the activities and experiments proposed in the projects that involve live, virulent Coccidioides species, Coccidioides immitis and Coccidioides posadasii, as they are classified as BSL3 pathogens. The other aim of the Core C is to utilize new methods to make targeted mutations in conserved genes in both species of Coccidioides, and to test the mutants for their ability to grow on media and in mice. The BSL3 facility will enable testing new hypotheses from Projects 1, 2, & 3 in mice, and to generate new hypotheses about immune responses from the results of mouse experiments proposed in Aim 1 to inform Project 3. The proposed studies in Aim 2 are directly related to Project 4 and will also shed light on the fungal genes that contribute to virulence, which may be new targets for therapeutics, and to identify host genes and response pathways that control immune responses to Coccidioides infection, which may reveal new therapeutic targets for biological agents.

摘要 模式生物核心的目标是开展拟议的生物危害研究项目 在项目1-4和核心本身，并保持两个不同菌株的培养物收集 Coccidioides的种类。这个核心有两个目标。一是开展所有提出的活动和实验 在涉及活的、有毒的球孢子菌属物种、粗球孢子菌和波萨达斯球孢子菌的项目中， 因为它们被归类为BSL 3病原体。Core C的另一个目的是利用新的方法， 在两种球孢子菌属中保守基因的突变，并测试突变体在 培养基和小鼠中。BSL 3设施将能够在小鼠中测试项目1、2和3的新假设， 从目标1中提出的小鼠实验结果中产生关于免疫应答的新假设 通知3号计划目标2中的拟议研究与项目4直接相关，也将阐明 真菌的致病基因，这可能是新的治疗目标，并确定宿主基因 以及控制球孢子菌感染免疫反应的反应途径，这可能揭示新的 生物制剂的治疗靶点。