Phenotypic and molecular correlates of parallel evolution of diapause and aging in African annual killifish

非洲一年生鳉鱼滞育和衰老平行进化的表型和分子相关性

• 批准号: 511530279
• 负责人: Professor Dr. Alessandro Cellerino, Ph.D.
• 金额: --
• 依托单位: Leibniz-Institut für Alternsforschung - Fritz-Lipmann-Institut e.V. (FLI)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/511530279?language=en
• 关键词: Phenotypic; molecular; correlates; parallel; evolution

The mechanisms of evolution are best studies in clades that independently evolve similar adaptations. At least three independent clades of Aplocheiloidei (killifishes) are adapted to ephemeral aquatic habitats cyclically generated by the monsoons and all show embryonic diapause and seasonal lifespan thereby representing a suitable model to investigate the molecular underpinnings of life-history traits evolution. The annual killifish Nothobranchius furzeri has become a laboratory model organism and is the shortest-lived vertebrate that can be cultured in captivity. Its genome is sequenced and transgenesis and genome-editing techniques are established and revealed new molecular mechanisms of diapause and aging. Therefore, annual killifishes represent unique models bridging evolutionary biology and biomedical sciences and molecular mechanisms can be identified by comparative analysis and then experimentally tested in N. furzeri. Among molecular mechanisms, regulation of RNA expression is the most widely investigated. However, proteins and not RNAs are the biologically active molecules. Global analysis of protein composition has been recently enabled by the advancement of mass-spectroscopy. By means of these technologies, we recently demonstrated 1) that protein and transcript regulation become uncoupled during N. furzeri aging. 2) that protein expression during aging and diapause are positively correletated and 3) that protein-transcript decoupling is prominent during diapause. The application has the objective to identify variation in protein expression that are associated into multiple clades with diapause and short lifespan.

进化机制最好是在独立进化出类似适应的进化枝中进行研究。至少有三个独立的分支Aplocheiloidei（鳉）适应短暂的水生栖息地周期性产生的季风和所有显示胚胎滞育和季节性寿命，从而代表了一个合适的模型来调查生活史性状进化的分子基础。一年生鳉鱼Nothobranchius furzeri已成为实验室模式生物，是可以人工养殖的最短寿命的脊椎动物。它的基因组测序和转基因和基因组编辑技术的建立，揭示了滞育和衰老的新分子机制。因此，一年生鳉鱼代表了连接进化生物学和生物医学科学的独特模式，其分子机制可以通过比较分析来确定，然后在N. furzeri。在分子机制中，RNA表达的调节是最广泛研究的。然而，蛋白质而不是RNA是生物活性分子。全球蛋白质组成的分析最近已经能够通过质谱的进步。通过这些技术，我们最近证明：1）蛋白质和转录调控在N。furzeri老化。2)衰老和滞育过程中蛋白质的表达呈正相关; 3）滞育过程中蛋白质-转录本解耦显著。本申请的目的是鉴定与滞育和短寿命的多个进化枝相关的蛋白质表达的变化。