Functional Characterization of Unconventional Myosin Motors

非常规肌球蛋白运动的功能表征

• 批准号: 51237887
• 负责人: Professor Dr. Dietmar J. Manstein
• 金额: --
• 依托单位: Institut für Biophysikalische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/51237887?language=en
• 关键词: Functional; Characterization; Unconventional; Myosin; Motors

Previous work allowed us to link structural alterations introduced by specific mutations into the myosin motor domain to the mechanism of chemomechamcal energy transduction and the in vivo and in vitro functions of class-1 and class-2 myosins. Within the context of the proposed work we aim to shift the main focus of our work from the Dictyostelium model system, which was used exclusively in previous work, to the study of mammalian myosins. Specifically, we will characterise the structural and functional properties of MyoG, which is produced during all stages of Dictyostelium development, and mammalian myosins belonging to classes 16 and 18. Class 16 myosins are most prominent in developing neural tissue and are likely to share some of their functional properties with class-1 myosins. Class 18 myosins are produced rather ubiquitously. They form heavy chain dimers and it is questionable whether they can act as molecular motors. All three types of myosins contain protein interaction domains outside the motor domain. Our long term goal in entering these studies is the elucidation of connections between motor mediated transport processes and chemical signalling events.

先前的工作使我们能够将由特定突变引入肌球蛋白马达结构域的结构改变与化学机械能量转导机制以及1类和2类肌球蛋白的体内和体外功能联系起来。在所提出的工作的背景下，我们的目标是转移我们的工作的主要重点从Dictyosteoblastoma模型系统，这是专门用于在以前的工作，哺乳动物肌球蛋白的研究。具体而言，我们将研究MyoG的结构和功能特性，MyoG是在网骨藻发育的所有阶段产生的，以及属于第16和18类的哺乳动物肌球蛋白。16类肌球蛋白在发育中的神经组织中最突出，并且可能与1类肌球蛋白共享它们的一些功能特性。18类肌球蛋白的产生相当普遍。它们形成重链二聚体，并且它们是否可以充当分子马达是有疑问的。所有三种类型的肌球蛋白都含有运动结构域之外的蛋白质相互作用结构域。我们进入这些研究的长期目标是阐明运动介导的运输过程和化学信号事件之间的联系。