Generierung monoklonaler Antikörper, Unterstützung der Proteinanalyse durch Massenspektrometrie und der Produktion rekombinanter Proteine in Insektenzellen

生成单克隆抗体，支持质谱蛋白质分析以及昆虫细胞中重组蛋白质的生产

• 批准号: 22177968
• 负责人: Professor Dr. Dietmar J. Manstein
• 金额: --
• 依托单位: Institut für Biophysikalische Chemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/22177968?language=en
• 关键词: Generierung; monoklonaler; Antik; rper; Unterst

Most projects within the research unit will require poly- and mononoclonal antibodies as essential tools to follow up specific project-re levant questions. Polyclonal antibodies can usually be generated by companies (such as Eurogentec or BioGENES) with acceptable quality, whereas the quality of many monoclonal antibodies, especially of those which are commercially available, is often non-satisfactory. Moreover, custom generation of monoclonal antibodies is by far too expensive to be funded within the individual projects. Therefore a centralised service project will be established for the generation of monoclonal antibodies. Other bottle necks in terms of detailed biochemical and structural analysis of isolated proteins and protein complexes concern rapid identification and characterisation of proteins by mass spectrometry and recombinant expression and production of proteins in insect cells using baculovirus. Since the project members have usually not established these techniques within their own laboratory and/or have no direct access to the quite expensive and often complex infrastructure, the research unit intends to establish a centralised service project, to provide the required technologies, i.e. monoclonal antibody generation, mass spectrometry and protein production in insect cells.

研究单位内的大多数项目将需要多克隆抗体和单克隆抗体作为跟进特定项目相关问题的基本工具。多克隆抗体通常可以由公司（如Eurogentec或BioGENES）以可接受的质量生产，而许多单克隆抗体的质量，特别是那些可商购的单克隆抗体的质量通常不令人满意。此外，单克隆抗体的定制生产成本太高，无法在单个项目中获得资金。因此，将建立一个集中服务项目，用于生产单克隆抗体。在分离的蛋白质和蛋白质复合物的详细生化和结构分析方面的其他瓶颈涉及通过质谱法快速鉴定和表征蛋白质以及使用杆状病毒在昆虫细胞中重组表达和生产蛋白质。由于项目成员通常没有在自己的实验室内建立这些技术和/或无法直接使用相当昂贵且往往复杂的基础设施，研究单位打算建立一个集中服务项目，以提供所需的技术，即单克隆抗体生成、质谱分析和昆虫细胞中的蛋白质生产。