Functional Characterisation of Myosin Motors (Funktionelle Charakterisierung von Myosinmotoren)

肌球蛋白运动的功能表征

• 批准号: 5175356
• 负责人: Professor Dr. Dietmar J. Manstein
• 金额: --
• 依托单位: Institut für Biophysikalische Chemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 1999
• 资助国家: 德国
• 起止时间: 1998-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5175356?language=en
• 关键词: Functional; Characterisation; Myosin; Motors; Funktionelle

The generation and characterisation of recombinant myosins will be one of the major contributions of our project to the programme. We will specifically design and optimise myosins and myosin fragments for use in kinetic, structural and cell biological studies. This approach will allow us to link structural alterations introduced with specific mutations to the in vivo and in vitro functions of the myosin motor. Major questions that can be addressed with this approach are: (1) How do mutations in the actin-binding face of the myosin motor affect the interaction with actin? (2) How do mutations in the actin-binding face alter events in other domains of the motor? (3) How is information communicated between the nucleotide-binding site, the actin binding-face, and the lever arm? (4) Can the enzymatic properties of the motor be altered in a predictable way by exchanging surface loops or structural modules with synthetic sequences? (5) How do the enzymatic properties of unconventional myosins differ from those of a myosin II?

重组肌球蛋白的产生和表征将是我们项目对该计划的主要贡献之一。我们将专门设计和优化肌凝蛋白和肌凝蛋白片段用于动力学，结构和细胞生物学研究。这种方法将使我们能够将特定突变引入的结构改变与肌球蛋白运动的体内和体外功能联系起来。这种方法可以解决的主要问题是：(1)肌凝蛋白马达肌动蛋白结合面突变如何影响肌动蛋白与肌动蛋白的相互作用？(2)肌动蛋白结合面的突变如何改变马达其他区域的事件？(3)核苷酸结合位点、肌动蛋白结合面和杠杆臂之间的信息是如何传递的？(4)马达的酶性质能否通过与合成序列交换表面环或结构模块而以可预测的方式改变？(5)非常规肌球蛋白的酶学性质与肌球蛋白II有何不同？