Nano-3D-printer - Upgrade

2014年在慕尼黑应用科学大学实施的纳米3d打印机目前正在光学技术、生物制造和微流体领域的几个研究项目中使用。作为第一代纳米3d打印机（双光子聚合），它具有高的空间分辨率，每条线约0.1 μm aber。但在我的书写速度也很低。一般来说，纳米打印需要好几个小时。对于复杂的物体，几天的时间并不罕见。在生物制造领域，这不仅限制了打印产品的数量，从而限制了可能的实验数量，而且从一开始就排除了从细胞外基质（ECM）蛋白质等生物材料生产复杂3D结构的可能性，因为所使用的生物树脂不允许打印时间长达数天。例如，在光学技术领域中，已经证明了每个直径为50 μm的涡旋具有良好的涡流特性。直径为m，满足光学要求。为了将涡旋用作光学元件，它的直径必须达到500 μm / s。在m.它的打印时间将超过一个星期的每个组件。在这个打印时间里，光刻胶有退化的风险，这一方面对打印质量有负面影响，但也会损坏使用的镜头。将纳米3d打印机升级为galvo扫描头，将打印速度提高了两到三个数量级。这将打印时间减少到几分钟。这不仅增加了可以进行的实验的数量，而且减少了打印所需的时间。与此同时，在几百微米范围内的更大的物体可以以相同的表面质量生产出来，例如，这为新的光学应用提供了机会。此外，需要复杂的3D支架结构的蛋白质进行生物制造。这在马丁斯里德mpi生物化学的可行性研究中得到了证明。然而，详细的调查是不可能的，因为那里的纳米3d打印机对非mpi成员严格限制。

The nano-3D printer implemented at Munich University of AppliedSciences in 2014 is currently being used in several research projectsin the field of optical technologies, biofabrication and microfluidics. Asthe first generation of nano-3D printers (2-photon polymerization), ithas a high spatial resolution of about 0.1 μm aber auch eine sehr geringe Schreibgeschwindigkeit. In m but also a very lowwriting speed. As a rule, nano prints take many hours. Forcomplicated objects, several days are not uncommon. In the field ofbiofabrication, this not only limits the number of printed products andthus the number of possible experiments, but also precludes theproduction of complex 3D structures from biological materials such asproteins of the extracellular matrix (ECM) from the outset, as the thebio-resins used do not allow printing times of several days. In the fieldof optical technologies, for example, it has been shown that a vortexwith a 50 μm aber auch eine sehr geringe Schreibgeschwindigkeit. In m diameter fulfills the optical requirements. In order to usethe vortex as an optical element, it would have to have a diameter of500 μm aber auch eine sehr geringe Schreibgeschwindigkeit. In m. Its printing time would be well over a week per component.With this printing time, there is a risk that the photoresists willdegenerate, which on the one hand has a negative impact on the printquality, but also damages the lenses used. Upgrading the nano-3Dprinter with a galvo scan head speeds up the processes by by two tothree orders of magnitude. This reduces the printing time to a fewminutes. This not only increases the number of possible experimentsthat can be carried out, but also reduces the time required for printing.At the same time, larger objects in the range of several hundredmicrometers can be produced with the same surface quality, whichopens new optical applications, for example. In addition, therequested complex 3D scaffold structures from proteins forbiofabrication.This was demonstrated in a feasibility study at the MPIfor Biochemistry in Martinsried. However, detailed investigations arenot possible there because access to the nano-3D printer there isseverely restricted for non-MPI members.