Time-resolved fluorescence and surface-enhanced Raman scattering spectroscopy microscope

时间分辨荧光和表面增强拉曼散射光谱显微镜

• 批准号: 515366497
• 金额: --
• 依托单位: Universität Hamburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/515366497?language=en
• 关键词: Time; resolved; fluorescence; surface; enhanced

Sensing of intracellular analytes (mostly located in endosomes/lysosomes) will be performed by loading cells with analyte-responsive dyes, optionally coupled to carrier particles, via endocytosis, microinjection, or membrane permeation. Intracellular analyte concentrations will then be determined by multiplexed read-out of the dye signals. In order to avoid crosstalk with the potential (here unwanted) pH-response of the dyes, the analyte-response of the dyes will be read-out laterally resolved either in the form of time-resolved fluorescence microscopy (FLIM) or surface-enhanced Raman scattering (SERS) microscopy. Multiplexed read-out of dyes in different cells within two- and three-dimensional co-culture systems will be facilitated by cell-labelling with SERS tags.

通过将细胞加载带有分析物反应性染料的细胞（可选地通过内吞作用，微注射或膜渗透）加载细胞，对细胞内分析物（主要位于内体/溶酶体）进行感测。然后，细胞内分析物浓度将通过染料信号的多路复用读数来确定。为了避免与染料的电势（此处不需要的）pH反应串扰，将以时间分解的荧光显微镜（FLIM）或表面增强的拉曼散射（SERS）显微镜的形式读取染料的分析物反应。两维共培养系统中不同细胞中染料的多重读数将通过带有SERS标签的细胞标记来促进。