Time-resolved fluorescence and surface-enhanced Raman scattering spectroscopy microscope

时间分辨荧光和表面增强拉曼散射光谱显微镜

• 批准号: 515366497
• 金额: --
• 依托单位: Universität Hamburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/515366497?language=en
• 关键词: Time; resolved; fluorescence; surface; enhanced

Sensing of intracellular analytes (mostly located in endosomes/lysosomes) will be performed by loading cells with analyte-responsive dyes, optionally coupled to carrier particles, via endocytosis, microinjection, or membrane permeation. Intracellular analyte concentrations will then be determined by multiplexed read-out of the dye signals. In order to avoid crosstalk with the potential (here unwanted) pH-response of the dyes, the analyte-response of the dyes will be read-out laterally resolved either in the form of time-resolved fluorescence microscopy (FLIM) or surface-enhanced Raman scattering (SERS) microscopy. Multiplexed read-out of dyes in different cells within two- and three-dimensional co-culture systems will be facilitated by cell-labelling with SERS tags.

细胞内分析物（主要位于内体/溶酶体中）的感测将通过经由内吞作用、显微注射或膜渗透用分析物响应性染料加载细胞来进行，所述分析物响应性染料任选地偶联至载体颗粒。然后通过染料信号的多路复用读出来确定细胞内分析物浓度。为了避免与染料的潜在（这里不希望的）pH响应的串扰，染料的分析物响应将以时间分辨荧光显微术（FLIM）或表面增强拉曼散射（Sers）显微术的形式被横向分辨地读出。在二维和三维共培养系统内的不同细胞中的染料的多重读出将通过用Sers标签进行细胞标记来促进。