Practical strategies for engineering the CO2-fixing enzyme, Rubisco, whose subunits are encoded in different subcellular compartments.

设计 CO2 固定酶 Rubisco 的实用策略，其亚基在不同的亚细胞区室中编码。

• 批准号: DP0450564
• 负责人: Prof Spencer Whitney
• 金额: $ 37.77万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2004
• 资助国家: 澳大利亚
• 起止时间: 2004-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0450564
• 关键词: Practical; strategies; engineering; CO2; fixing

My recent replacement of the plant CO2-fixing enzyme, Rubisco, with a less efficient bacterial version, with a single type of subunit encoded by a single gene, demonstrated the feasibility of replacing Rubisco. This encourages ongoing attempts to replace plant Rubisco with more efficient versions that would allow the plants to grow with less water, fertiliser or light. The most efficient Rubiscos are more complex, with two different types of subunits which, in plants, are encoded in different subcellular compartments (nucleus and plastid). This proposal addresses the challenges associated with complementary engineering both genomes to substitute foreign Rubiscos into higher-plant chloroplasts.

最近，我用一种效率较低的细菌版本取代了植物的二氧化碳固定酶Rubisco，这种细菌版本具有由单一基因编码的单一类型的亚基，证明了取代Rubisco的可行性。这鼓励人们不断尝试用更有效的版本取代植物Rubisco，使植物在更少的水、肥料或光照下生长。最有效的Rubiscos更复杂，有两种不同类型的亚基，在植物中，在不同的亚细胞区室（细胞核和质体）中编码。该提案解决了与互补工程两个基因组取代外来Rubiscos到高等植物叶绿体相关的挑战。