Controlling the rate of transcription and translation of Rubisco transgenes effectively in higher-plant plastids.

在高等植物质体中有效控制 Rubisco 转基因的转录和翻译速率。

• 批准号: DP0342560
• 负责人: Prof Spencer Whitney
• 金额: $ 1.37万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0342560
• 关键词: Controlling; rate; transcription; translation; Rubisco

Genetic transformation of the circular genome of the plastids provides a containable means for modifying plant growth by manipulating photosynthesis. Although the transformation mechanism is precise, predicting the level of foreign gene expression is difficult because the amounts of messenger RNA and protein produced by foreign genes in plastids varies widely, even when the protein assembles without difficulty. This project will devise strategies for controlling this variability that will facilitate attempts to exploit plastid transformation for transplanting better versions of the photosynthetic CO2-fixing enzyme, Rubisco, into plants to improve their growth efficiency in terms of water, fertiliser and light use.

叶绿体环状基因组的遗传转化为通过操纵光合作用改变植物生长提供了一种可容纳的手段。尽管转化机制是精确的，但预测外源基因的表达水平是困难的，因为外源基因在叶绿体中产生的信使RNA和蛋白质的数量差异很大，即使蛋白质组装起来没有困难。该项目将制定控制这种变异性的策略，以促进利用叶绿体转化将更好版本的光合作用二氧化碳固定酶Rubisco移植到植物中，以提高它们在水、肥料和光能利用方面的生长效率。