Energy driven processes in anaerobic bacteria

厌氧细菌的能量驱动过程

• 批准号: 5172012
• 负责人: Professor Dr. Wolfgang Buckel
• 金额: --
• 依托单位: Laboratory for Microbial Biochemistry
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 1999
• 资助国家: 德国
• 起止时间: 1998-12-31 至 2005-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5172012?language=en
• 关键词: Energy; driven; processes; anaerobic; bacteria

The goal of this proposal is to show whether H2-formation from glutamate via NADH in the strict anaerobe bacterium Acidaminococcus fermentans is driven by a H+ or Na+ gradient. In order to understand the mechanism of this reversed electron transport, two membrane enzymes, a hydrogenase and a NADH dehydrogenase, which probably participate in this reaction, will be thoroughly characterised by biochemical, genetic and biophysical techniques. The purified enzymes will be incorporated into phospholipid vesicles, in which artificial ion gradients should induce H2-formation from NADH. The thereby achieved amplification of the reductive power of NADH (E°'= -320 mV) to the level of reduced ferredoxin (E°' = 450 mV) could be of general importance for anaerobes. Reduced ferredoxin is inter alia required for pyruvate synthesis from acetyl-CoA, nitrogen fixation, reduction of benzoyl-CoA and probably for the initiation of the dehydrations of (R)-lactyl-CoA and (R)-2-hydroxyglutaryl-CoA.

该提议的目标是显示在严格厌氧细菌发酵氨基酸球菌中通过NADH从谷氨酸形成H2是否由H+或Na+梯度驱动。为了了解这种反向电子传递的机制，两种膜酶，氢化酶和NADH脱氢酶，可能参与这一反应，将通过生物化学，遗传学和生物物理学技术进行彻底表征。纯化的酶将被纳入磷脂囊泡，其中人工离子梯度应诱导从NADH的H2-形成。由此实现的将NADH的还原能力（E ° '=-320 mV）放大至还原的铁氧还蛋白水平（E °'= 450 mV）对于厌氧菌可能具有普遍重要性。还原的铁氧还蛋白尤其是从乙酰-CoA合成丙酮酸、固氮、还原苯甲酰基-CoA所需的，并且可能是（R）-乳酰-CoA和（R）-2-羟基戊二酰-CoA脱水起始所需的。