Structure of bakterial ABC-transporters for maltose/maltodextrines
麦芽糖/麦芽糊精细菌 ABC 转运蛋白的结构
基本信息
- 批准号:5172202
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Priority Programmes
- 财政年份:1999
- 资助国家:德国
- 起止时间:1998-12-31 至 2010-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The family of ABC transport systems comprises a diverse class of transport proteins that couple the energy of ATP hydrolysis to the translocation of solutes across biological membranes. Members of the family have been identified in organisms belonging to each of the three major kingdoms, some of them with medical relevance (antibiotic and multidrug resistance, cystic fibrosis). The molecular mechanism by which these proteins exert their functions is still poorly understood, mainly due to the lack of tertiary structural informations. By using the well-studied ABC transport system for maltose (Ma1FGK2) of salmonella typhimurium as a mode, we will contribute to the investigations of the field. First, we will solve the structure of the ATP-binding subunit Ma1K by X-ray diffraction analysis of available crystals. Complementary to these studies we will determine the solution structure of a C-terminal Ma1K fragment by NMR. In a second set of experiments we will attempt to obtain 2D- and 3D-crystals of the purified Ma1FGK2 complex. Analysis of the preparation by electron microscopy and the production of 2D crystals will provide additional structural informations. Finally, a homologous transport system from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius will be studied as an alternative, thereby taking advantage from its increased protein stability in crystallization experiments.
ABC转运系统家族包括多种转运蛋白,其将ATP水解的能量与溶质跨生物膜的易位偶联。该家族的成员已在属于三个主要王国的生物体中鉴定,其中一些具有医学意义(抗生素和多药耐药性,囊性纤维化)。这些蛋白质发挥其功能的分子机制仍然知之甚少,主要是由于缺乏三级结构信息。通过使用已被充分研究的ABC转运系统麦芽糖(Ma 1 FGK 2)的鼠伤寒沙门氏菌作为一个模式,我们将有助于该领域的调查。首先,我们将通过可用晶体的X射线衍射分析来解决ATP结合亚基Ma 1 K的结构。作为这些研究的补充,我们将通过NMR确定C-末端Ma 1 K片段的溶液结构。在第二组实验中,我们将尝试获得纯化的Ma 1FGK 2复合物的2D和3D晶体。通过电子显微镜和2D晶体的生产来分析制备将提供额外的结构信息。最后,同源运输系统的嗜热嗜酸细菌Alicyclobacillus acidocaldarius将作为一种替代方案进行研究,从而利用其增加的蛋白质稳定性在结晶实验。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Professor Dr. Erwin Schneider其他文献
Professor Dr. Erwin Schneider的其他文献
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{{ truncateString('Professor Dr. Erwin Schneider', 18)}}的其他基金
Analysis of coupling between substrate binding and ATP hydrolysis in canonical homo- and heterodimeric amino acid ABC import systems
经典同二聚体和异二聚体氨基酸 ABC 输入系统中底物结合与 ATP 水解之间的耦合分析
- 批准号:
315832426 - 财政年份:2016
- 资助金额:
-- - 项目类别:
Research Grants
Comparative analysis of the dynamics of the binding protein-dependent histidine ABC import system and the ECF-type biotin transporter
结合蛋白依赖性组氨酸ABC输入系统和ECF型生物素转运蛋白的动力学比较分析
- 批准号:
137770687 - 财政年份:2009
- 资助金额:
-- - 项目类别:
Research Grants
Acarbose, the first example of a carbophor?
阿卡波糖,卡波佛的第一个例子?
- 批准号:
12815386 - 财政年份:2005
- 资助金额:
-- - 项目类别:
Research Grants
Protein-Protein interactions and conformational changes of an ATP-binding-cassette (ABC) transporter: the maltose transport system of Salmonella typhimurium
ATP 结合盒 (ABC) 转运蛋白的蛋白质-蛋白质相互作用和构象变化:鼠伤寒沙门氏菌的麦芽糖转运系统
- 批准号:
5419101 - 财政年份:2004
- 资助金额:
-- - 项目类别:
Research Grants
ATP-getriebene Transportsysteme für Maltose/Maltodextrine (ABC-Transporter) bei Mikroorganismen
微生物中 ATP 驱动的麦芽糖/麦芽糖糊精转运系统(ABC 转运蛋白)
- 批准号:
5246878 - 财政年份:1995
- 资助金额:
-- - 项目类别:
Research Grants
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细菌翻译中硒代半胱氨酸掺入的分子机制
- 批准号:
5425290 - 财政年份:2004
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