An Experimental Study for the Permeability of Oral Mucosa

口腔粘膜渗透性的实验研究

• 批准号: 59570843
• 负责人: ENOMOTO Shoji
• 金额: $ 1.02万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59570843/
• 关键词: Oral Mucosa; Permeability; Iontophoresis; クロマトグラフィー; イオン導入; オーラルマイクロスコープ

1. The permeability of rat oral mucosa was investigated by use of autoradiography. We applied tritium - labeled Triamcinolon CMC ointment to buccal mucosa, tongue and the floor of the mouth. We obtained the results that Triamcinolon was penetrated time dependently from the surface of epithelial layer to the submucosal layer and muscle layer and that there were regional differences of penetration.2. The concentration of Triamcinolon in the rat tongue tissue was investigated. Triamcinolon acetonide oral film was applied and after 15, 30, 60 and 120 min., Triamcinolon in the tongue tissue was measured by the chromatography. We obtained the results that the dose of Triamcinolon were increased in the epithelium just under the film time dependently, and that each Triamcinolon was not so diffused arround the film region.3. Iontophoresis was tried on the oral lesions, such as lichen planus, leukoplakia or cancer. There were some effective cases of lichen・planus by the use of dexamethasone and a few cases of leukoplakia were effective by the use of anti-tumor agent, BLM, but no cases of cancer were effective in our study.4. We tried to use oral microscope to observe the permeability of oral mucosa. Normal human oral mucosa was stained by toruidine-blue or cresyl-violet. We obtained the results that there were many polygonal shaped cells having oral nucleus in the surface layer of the epithelium. The methods of the fixation of the scope and staining of the living body were discussed.

1.用放射自显影法研究了大鼠口腔粘膜的通透性。我们将氚标记的曲安奈德CMC软膏应用于颊粘膜、舌和口底。结果表明：曲安奈德从上皮层表面向黏膜下层和肌层渗透呈时间依赖性，且渗透存在区域差异.研究了大鼠舌组织中曲安奈德的浓度。应用曲安奈德口腔膜剂，并在15、30、60和120分钟后，用色谱法测定舌组织中曲安奈德的浓度。结果表明，曲安西龙在膜下上皮细胞中的剂量随时间增加而增加，但曲安西龙在膜周围的扩散并不明显.对口腔扁平苔藓、白斑、口腔癌等病变进行离子导入治疗。地塞米松治疗扁平苔藓有部分有效病例，抗肿瘤药物博莱霉素治疗白斑有部分有效病例，而肿瘤治疗无有效病例.我们尝试用口腔显微镜观察口腔粘膜的通透性。正常人口腔粘膜分别用妥瑞定蓝和甲酚紫染色。结果表明，在上皮细胞的表层有许多多边形细胞，具有口核。讨论了显微镜的固定方法和活体的染色方法。