权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Establishment of Mandibulor Reconstruction with Bone Merphogenetic Protein

骨形态发生蛋白重建下颌骨的建立

基本信息

批准号：
05557089
负责人：
ENOMOTO Shoji
金额：
$ 8.32万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05557089/
关键词：
BMP rhBMP BMP Carrier Repair of alveolar bone Mandibular reconstruction BMP rh-BMP PGLA 人口骨担体人工骨骨誘導機能的顎骨再建

项目摘要

It is necessary to develop the carrier of Bone Morphogenetic Protein (BMP) for its clinical use. First we implanted the biodegradable porous polymer disks in rat skull defects, and investigated the effects radiographycally and histologically. The implant disks were prepared using a freeze-drying technique by incorporating bovine bonermorphogenetic protein (BMP) in poly (glycolic acid-co-lactic acid) (PGLA). The PGLA disks with and without BMP were implanted in the skull defects created by trephine bar. A quantitative radiographic analysis showed significantly thicker radiopacity for the disks with BMP than those without BMP.Histologically the defects were completely replaced by new bone with the disks BMP-incorporated after 4 weeks, but by fibrous connective tissue with those using PGLA alone without BMP.The results suggest that BMP-incorporated PGLA implant has an osteoinductive capability and potential as bone graft substitutes.Secondary, we investigated the effect of recombinant human BMP (rh-BMP) with carrier to mandibular bone defects in dogs. We created the defects on both sides of the alveolar bone. The size of the defects was 5X 10X 15mm, and we also used the biodegradable polymer sheets after marginal ressection. The results were that new bone formation in the defect could be observed 2 weeks after operation although it was entirely not observed in the side without rhBMP,and the defect with rhBMP was occupied with new bone after 8 weeks. Furthermore the effects depended on time and dose of rhBMP.From these results, we assumed that rhBMP,with biodegradable and porous polymer, can be applied to our clinic, and we expected to use for mandibular bone re-costruction after marginal and segmental ressection.

骨形态发生蛋白（BMP）载体的研制是其临床应用的必要条件。首先，我们将生物可降解多孔聚合物圆盘植入大鼠颅骨缺损中，并通过放射学和组织学观察其效果。通过将牛骨形态发生蛋白（BMP）掺入聚（乙醇酸-共-乳酸）（PGLA）中，使用冷冻干燥技术制备植入物圆盘。将含BMP和不含BMP的PGLA盘植入环钻造成的颅骨缺损中。组织学观察显示，BMP复合物植入4周后缺损完全被新骨替代，而单纯PGLA植入4周后缺损被纤维结缔组织替代。结果表明，BMP复合物具有骨诱导能力，可作为骨移植替代物。本实验研究了重组人骨形态发生蛋白（rh-BMP）复合载体对犬下颌骨缺损的修复作用。我们在两侧牙槽骨上造成了缺损。缺损的大小为5 × 10 × 15 mm，我们也在边缘切除后使用可生物降解聚合物片。结果显示：术后2周缺损区有新骨形成，而未植入rhBMP的一侧完全无新骨形成，植入rhBMP的一侧在术后8周缺损区有新骨形成。结果表明，rhBMP具有生物可降解性和多孔性，可以应用于临床，并有望应用于下颌骨边缘和节段性切除后的骨重建。