Changes in neuroactive substances in trigeminal motor nucleus after peripheral nerve injury

周围神经损伤后三叉神经运动核神经活性物质的变化

• 批准号: 08835023
• 负责人: WADA Yo
• 金额: $ 1.41万
• 依托单位: HYOGO COLLEGE OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08835023/
• 关键词: trigeminal motor nucleus; calcitonin gene-related peptide; in situ hybridization; masseteric nerve; peripheral nerve injury; in situ ハイブリダイゼーション法

The aim of this study was to examine the change in alpha and beta calcitonin gene related peptide mRNA expression in trigeminal motor nucleus following peripheral nerve injury. Rats received the transection of left masseteric nerve under deep anesthesia. After 1-56 postoperative days, the brainstem was dissected out for in situ hybridization. Trigeminal motor nucleus showed beta-CGRP mRNA expression constitutively and no alpha-CGRP mRNA.After transection of unilateral masseteric nerve, trigeminal motor nucleus express alpha-CGRP mRNA dramatically and continued high level at least 8 weeks after nerve injury. beta-CGRP mRNA showed small increase compared to alpha-CGRP mRNA.These data suggest that trigeminal motor nucleus showed differential expression of alpha-and beta-CGRP mRNA after peripheral nerve injury.

本研究的目的是观察周围神经损伤后三叉神经运动核内降钙素基因相关肽α和β mRNA表达的变化。大鼠在深度麻醉下切断左侧咬肌神经。术后1-56天，取脑干进行原位杂交。三叉神经运动核内有β-CGRP mRNA的组成性表达，无α-CGRP mRNA的表达，咬肌神经切断后，三叉神经运动核内α-CGRP mRNA的表达显著增加，并在咬肌神经损伤后8周持续高水平表达。结果表明，周围神经损伤后，三叉神经运动核内α-和β-CGRP mRNA表达有差异。

项目成果

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Fukuoka,T et al: "Etpression of β-calcitonin geve-related peptiole in atotowized rubrospinal neurons and the effect of brain derived neurotropho factor" Brain Research. 767. 250-258 (1997)

Fukuoka，T 等人：“β-降钙素 geve 相关肽在原子化红核脊髓神经元中的表达以及脑源性神经营养因子的作用”《大脑研究》767. 250-258 (1997)。

Fukuoka, T et al: "Expression of β-calcitonin gene-related peptide in cototonized rubrospinal neurons and the effect of brains derived pasistiqte factor" Brain Research. 767. 250-258 (1997)

Fukuoka，T 等人：“β-降钙素基因相关肽在 cototonized 红核脊髓神经元中的表达以及脑源性 pasistiqte 因子的作用”Brain Research 767. 250-258 (1997)。