Development of assessment method for complex aquatic pollution using xenobiotic metabolic enzyme in liver of aquatic mammals

利用水生哺乳动物肝脏中的异生物质代谢酶评估复杂水生污染的方法

• 批准号: 09306021
• 负责人: KAZUSAKA Akio
• 金额: $ 17.6万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09306021/
• 关键词: largha seals; ribbon seals; chlorinated hydrocarbon; cytochrome P450 in liver; thyroid function; thyroxine; thyronine; 内分泌攪乱物質; トリブチルスズ; ビスフェノール A; ラット; 中枢神経系; 精巣上体の発育不良; 血中チロキシン濃度の低下; 海棲哺乳動物; チトクロームP450; 環境汚染; ゴマアザラシ; 水棲哺乳動物; 異物代謝酵素

The correlation between the residual chlorinated hydrocarbon levels and the cytochrome P450 expression levels in livers and the thyroid function were studied in largha seals and ribbon seals from the coast of Hokkaido. The largha seals showed the significant levels of residual non-ortho mono-ortho coplanar PCB homologues in their epidermis with the hepatic enzymatic activity of ethoxyresorfin deethylation (EROD). The Western blot analysis indicated that the coplanar PCB homologue levels were related with those of P4501A-like protein. The ribbon seals, on the other hand, did not give such correlations. These results suggested that coplanar PCB induces P4501A-like enzyme in the largha seals, which is basedon the specific mechanism in this species.The quantities of total thyroxine, free thyroxine, total tri-iodo thyronine and free tri-iodo thyronine were measured in both seal blood withthose ages. The T3 was were found to correlated negatively with most chlorinated organic compounds. Especially, the PCB170 and PCB18O gave the significant and negative correlation with total T3 . Though the thyroid hormone is generally known to decrease by the aging, the values observed here exceeded them. It was anticipated that there is the serious effect for growth and reproduction of seals.

在北海道海岸的大量密封和丝带密封中研究了残留的氯化烃水平与细胞色素P450表达水平与甲状腺功能之间的相关性。大型密封件显示了其表皮中残留的非官方单核Coclanar PCB同源物的显着水平，其乙氧基雷神脱乙基化（EROD）的肝酶促活性具有显着水平。蛋白质印迹分析表明，共面PCB同源物水平与P4501a样蛋白的同源水平相关。另一方面，色带密封没有给出这种相关性。这些结果表明，共面PCB在largha密封剂中诱导P4501a样酶，这是该物种中的特定机制。总甲状腺素，游离甲状腺素，总三 - 碘甲状腺素和自由三碘二氧化碳的含量在两种密封血液中均应测量。发现T3与大多数氯化有机化合物呈负相关。特别是，PCB170和PCB18O与总T3产生了显着和负相关。尽管通常已知甲状腺激素会因衰老而降低，但此处观察到的值超过了它们。预计海豹的生长和繁殖会有严重的影响。

项目成果

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Yoon, S.、Kazusaka, A.、Fujita, S.：“患有肝炎的 Long-Evance 肉桂 (LEC) 大鼠肝膜中甘油二酯的积累。”Cancer lettr.. 151. 19-24 (2000)

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