Development of assessment method for complex aquatic pollution using xenobiotic metabolic enzyme in liver of aquatic mammals

利用水生哺乳动物肝脏中的异生物质代谢酶评估复杂水生污染的方法

• 批准号: 09306021
• 负责人: KAZUSAKA Akio
• 金额: $ 17.6万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09306021/
• 关键词: largha seals; ribbon seals; chlorinated hydrocarbon; cytochrome P450 in liver; thyroid function; thyroxine; thyronine; 内分泌攪乱物質; トリブチルスズ; ビスフェノール A; ラット; 中枢神経系; 精巣上体の発育不良; 血中チロキシン濃度の低下; 海棲哺乳動物; チトクロームP450; 環境汚染; ゴマアザラシ; 水棲哺乳動物; 異物代謝酵素

The correlation between the residual chlorinated hydrocarbon levels and the cytochrome P450 expression levels in livers and the thyroid function were studied in largha seals and ribbon seals from the coast of Hokkaido. The largha seals showed the significant levels of residual non-ortho mono-ortho coplanar PCB homologues in their epidermis with the hepatic enzymatic activity of ethoxyresorfin deethylation (EROD). The Western blot analysis indicated that the coplanar PCB homologue levels were related with those of P4501A-like protein. The ribbon seals, on the other hand, did not give such correlations. These results suggested that coplanar PCB induces P4501A-like enzyme in the largha seals, which is basedon the specific mechanism in this species.The quantities of total thyroxine, free thyroxine, total tri-iodo thyronine and free tri-iodo thyronine were measured in both seal blood withthose ages. The T3 was were found to correlated negatively with most chlorinated organic compounds. Especially, the PCB170 and PCB18O gave the significant and negative correlation with total T3 . Though the thyroid hormone is generally known to decrease by the aging, the values observed here exceeded them. It was anticipated that there is the serious effect for growth and reproduction of seals.

研究了北海道沿岸的长吻海豹和带状海豹肝脏中氯代烃残留水平与细胞色素P450表达水平及甲状腺功能之间的相关性。海狗的表皮中残留的非邻位单邻位共面PCB同系物的显着水平与乙氧基间苯二酚脱乙基（EROD）的肝酶活性。Western blot分析表明，共平面PCB同源物的水平与P4501A样蛋白的水平相关。另一方面，带状密封件没有给出这样的相关性。结果表明，PCB共面刺激诱导了海狗体内P4501A样酶的产生，其机制是海狗特有的。T3与大多数氯代有机物呈负相关。其中PCB170和PCB18O与总T3呈显著负相关。尽管众所周知甲状腺激素会随着年龄的增长而下降，但这里观察到的值超过了它们。预计对海豹的生长和繁殖有严重影响。

项目成果

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Yamamoto, H. 等人：“Long-Evance 肉桂 (LEC) 大鼠体内脂质过氧化增强的机制”Arch.

Hoshi,H.: "Organochlorine pesticides and polychlorinated biphenyl congeners in wild terrestrial mamals and binds" Chemosphere. 36・15. 3211-3221 (1998)

Hoshi, H.：“野生陆生哺乳动物中的有机氯农药和多氯联苯同系物及其结合”，Chemosphere 36・15 (1998)。

Yasunaga,G.,Watanabe,Prudente,M.,Tanabe,S., et al.: "Trace elements accumulation in waders from Asia"Toxicol.Environ.Chem.. 77. 75-92 (2000)

Yasunaga,G.,Watanabe,Prudente,M.,Tanabe,S., et al.:“亚洲涉水鸻体内微量元素的积累”Toxicol.Environ.Chem.. 77. 75-92 (2000)

藤田正一編: "毒性学"朝倉書店. 300 (1999)

藤田翔一主编：《毒理学》朝仓书店 300 (1999)。

Yoon, S., Kazusaka, A., Fujita, S.: "Accumulation of diacylglycerole in the liver membrane of Long-Evance cinnamon (LEC) rat with hepatitis."Cancer lettr.. 151. 19-24 (2000)

Yoon, S.、Kazusaka, A.、Fujita, S.：“患有肝炎的 Long-Evance 肉桂 (LEC) 大鼠肝膜中甘油二酯的积累。”Cancer lettr.. 151. 19-24 (2000)