ECHOTOXICOLOGY OF ULTRA VIOLET RADIATION ON EARTH ENHANCED BY DEPLETION OF STRATOSPHERIC OZONE

平流层臭氧消耗增强的地球紫外线辐射的回声毒理学

• 批准号: 05453199
• 负责人: KAZUSAKA Akio
• 金额: $ 4.48万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05453199/
• 关键词: Echotoxicology; Stratospheric ozone; Chloroplast; Chlorophyll; Singlet oxygen; Luminescence spectrum of chlorophyll; Peroxidation of thyrakoid membrane; Induction of protease; 植物葉緑体; クロロフィル類; イソフラボングルコサイドの蓄積; 穀物種子; エリシター活性の誘導

The depletion of of stratospheric ozone is considered to enhance UV radiation on the earth and to destroy its echosystem. Echotoxicolgy of UV radiation was studied for plants.A.The chloroplast fraction of a clover, Trifolium, was found to catalyze the demethylation of imipramine for its starting period. Chlrophyll was suggested to be an active compornent and to lose its catalytic activity spontaneously.B.A commercial chlorophyll a was used for catalytic demethylation of imipramine under radiation of light above 500nm, indicating that it produces singlet oxygen as a reation intermediate.C.In interest to utilize chlorophyll a as photocatalyst, it was found to be stable in nonpolar solvent but decomposed partially on MgO.Chlorophyll a impregnated on MgO gave a hydroxy radical under visible light, indicating its usefulness as photocatalyst.D.Spinach thyarkoid membrane was radiated by UV-A light in interest for its photodamage to plants. The radiation did not affect the amount of chlorophyll contained in it, but its luminescence spectra was changed indicating the photostress of thyrakoid membrane. Photoformation of singlet oxygen was observed in radiation of UV-A,but was deactivated with radiation time. Protease inhibitor stopped its deactivation, suggesting an induction of some kind of protease by UV-A radiation.

平流层臭氧的消耗被认为会增强地球上的紫外线辐射，并破坏地球的生态系统。研究了紫外光辐射对植物的生态毒理作用。A.三叶草的叶绿体部分在其起始阶段催化丙咪胺的脱甲基化。一种商品的叶绿素a在500 nm以上的光照下催化丙咪胺的脱甲基化反应，表明它能产生单线态氧作为反应中间体。为了利用叶绿素a作为光催化剂，发现它在非极性溶剂中很稳定，但在氧化镁上部分分解。辐射对类囊体膜中的叶绿素含量没有影响，但其发光光谱发生了变化，表明类囊体膜的光胁迫。在UV-A辐射下观察到单线态氧的光形成，但随着照射时间的延长而失活。蛋白水解酶抑制剂停止了其失活，表明UV-A辐射诱导了某种蛋白水解酶的产生。

项目成果

S.Hatakeyama: "Paradoxical effect of Sudan III on the in vivo and in vitro genotoxicity elicited by 7,12-dimethylbenz (a)-anthracen." J.Biochem.Toxicol.10. 143-149 (1995)

S.Hatakeyama：“苏丹 III 对 7,12-二甲基苯并 (a)-蒽引起的体内和体外遗传毒性的矛盾作用。”

T.Yamamoto: "Purification and chracterization of a from of cytochrom P-450 from bear liver microsomes." Biochem.Pharmacol.(in press). (1995)

T.Yamamoto：“熊肝微粒体细胞色素 P-450 的纯化和表征。”

Hatakeyama S.: "Paradoxical effect of Sudan III on the in vivo and in vitro genotoxicity elicited by 7,12-dimethylbenz (a) anthracen." J.Biochem. Toxicol.10. 143-149 (1995)

Hatakeyama S.：“苏丹 III 对 7,12-二甲基苯（a）蒽引起的体内和体外遗传毒性的矛盾作用。”

Hoshi H.: "Deblisoquine/sparteine-type polymorphic drug metabolism in wild clethrionomys rufocanus." Drug Metabol. Dispos.23. 1301-1303 (1995)

Hoshi H.：“野生 clehrionomys rufocanus 中的 Deblisoquine/sparteine 型多态药物代谢。”

S.Hatakeyama: "Paradoxical effect of Sudan III on the in vivo and in vitro genotoxicity elicited by 7,12-dimethy lbenz(a)-anthracen." J.Biochem.Toxicol.10. 143-149 (1995)

S.Hatakeyama：“苏丹 III 对 7,12-二甲基苯并(a)-蒽引起的体内和体外遗传毒性的矛盾作用。”