ECHOTOXICOLOGY OF ULTRA VIOLET RADIATION ON EARTH ENHANCED BY DEPLETION OF STRATOSPHERIC OZONE

平流层臭氧消耗增强的地球紫外线辐射的回声毒理学

• 批准号: 05453199
• 负责人: KAZUSAKA Akio
• 金额: $ 4.48万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05453199/
• 关键词: Echotoxicology; Stratospheric ozone; Chloroplast; Chlorophyll; Singlet oxygen; Luminescence spectrum of chlorophyll; Peroxidation of thyrakoid membrane; Induction of protease; 植物葉緑体; クロロフィル類; イソフラボングルコサイドの蓄積; 穀物種子; エリシター活性の誘導

The depletion of of stratospheric ozone is considered to enhance UV radiation on the earth and to destroy its echosystem. Echotoxicolgy of UV radiation was studied for plants.A.The chloroplast fraction of a clover, Trifolium, was found to catalyze the demethylation of imipramine for its starting period. Chlrophyll was suggested to be an active compornent and to lose its catalytic activity spontaneously.B.A commercial chlorophyll a was used for catalytic demethylation of imipramine under radiation of light above 500nm, indicating that it produces singlet oxygen as a reation intermediate.C.In interest to utilize chlorophyll a as photocatalyst, it was found to be stable in nonpolar solvent but decomposed partially on MgO.Chlorophyll a impregnated on MgO gave a hydroxy radical under visible light, indicating its usefulness as photocatalyst.D.Spinach thyarkoid membrane was radiated by UV-A light in interest for its photodamage to plants. The radiation did not affect the amount of chlorophyll contained in it, but its luminescence spectra was changed indicating the photostress of thyrakoid membrane. Photoformation of singlet oxygen was observed in radiation of UV-A,but was deactivated with radiation time. Protease inhibitor stopped its deactivation, suggesting an induction of some kind of protease by UV-A radiation.

平流层臭氧的耗竭被认为可以增强地球上的紫外线辐射并破坏其回声系统。研究了紫外线辐射的回声毒性。认为chlrophrophyll是一种活跃的组合物，并自发地失去其催化活性。B。在500nm上方的光的辐射下，使用商业叶绿素a用于催化丙氨酸的催化脱甲基化，这表明它会产生单粒氧气作为旋律中的杂物。但在MGO上部分分解。氯植物在MGO上浸渍的绿色植物在可见光下产生了羟基自由基，表明其作为光催化剂的有用性。辐射不影响其中含有的叶绿素的量，但其发光光谱已更改，表明甲状腺膜的光子。在UV-A的辐射中观察到单线氧的光子化，但用辐射时间停用。蛋白酶抑制剂停止了其失活，表明通过UV-A辐射诱导某种蛋白酶。

项目成果

T.Yamamoto: "Purification and chracterization of a from of cytochrom P-450 from bear liver microsomes." Biochem.Pharmacol.(in press). (1995)

T.Yamamoto：“熊肝微粒体细胞色素 P-450 的纯化和表征。”

S.Hatakeyama: "Paradoxical effect of Sudan III on the in vivo and in vitro genotoxicity elicited by 7,12-dimethylbenz (a)-anthracen." J.Biochem.Toxicol.10. 143-149 (1995)

S.Hatakeyama：“苏丹 III 对 7,12-二甲基苯并 (a)-蒽引起的体内和体外遗传毒性的矛盾作用。”

Hatakeyama S.: "Paradoxical effect of Sudan III on the in vivo and in vitro genotoxicity elicited by 7,12-dimethylbenz (a) anthracen." J.Biochem. Toxicol.10. 143-149 (1995)

Hatakeyama S.：“苏丹 III 对 7,12-二甲基苯（a）蒽引起的体内和体外遗传毒性的矛盾作用。”

S.Hatakeyama: "Paradoxical effect of Sudan III on the in vivo and in vitro genotoxicity elicited by 7,12-dimethy lbenz(a)-anthracen." J.Biochem.Toxicol.10. 143-149 (1995)

S.Hatakeyama：“苏丹 III 对 7,12-二甲基苯并(a)-蒽引起的体内和体外遗传毒性的矛盾作用。”

Hoshi H.: "Deblisoquine/sparteine-type polymorphic drug metabolism in wild clethrionomys rufocanus." Drug Metabol. Dispos.23. 1301-1303 (1995)

Hoshi H.：“野生 clehrionomys rufocanus 中的 Deblisoquine/sparteine 型多态药物代谢。”