DEVELOPMENT OF CYTOCHROME P-450 PROCESS FOR THE STUDY OF TOXICOLOGY WITHOUT USING ANIMALS

一些农用药品、药品、食品添加剂或化工产品作为环境激素对环境的污染越来越明显。然而，目前还没有建立起有关它们的毒理学评价技术。特别是以细胞色素P450为代谢酶激活的代谢物的毒性评价技术较少。最近有人指出，许多化学物质应该重新评估。因此，有必要开发新的技术来评价多种化学物质。因此，本项目关注的是开发细胞色素P450工艺，用于不使用小鼠或大鼠等动物的毒理学研究。在实验室(1)、自然环境(2)、分子生物学技术(3)或光谱技术(4)中，研究了细胞色素P-450对异种生物的酶促分析。每个结果都发表在几本杂志上。需要强调的是，光谱学方法是无创评估的新技术。

It is getting clear that some agricultural medicine, medicine, food additive or chemical product contaminates environment as an environmental hormone. Toxicological assessment technique, however, has not established about them. Especially there is a few technology to estimate toxicity of metabolites, which are activated by metabolic enzyme as cytochrome P450. It is pointed out recently that many chemicals should be estimated again. Therefore it is necessary to develop new technique to assess many chemicals. The present project is thus concerned with the development of cytochrome P450 process for the study of toxicology without using animals as mice or rats. Several experiments have been carried out to study an enzymatic analysis of cytochrome P-450 to xenobiotics in laboratory (1), in natural field (2), expressed by molecular biological technique (3), or using spectroscopic technique (4). Each result has been published in several journal. It should be stressed that new technique has been developed in noinvasive assessment to use spectroscopic method.