DEVELOPMENT OF CYTOCHROME P-450 PROCESS FOR THE STUDY OF TOXICOLOGY WITHOUT USING ANIMALS

一些农药、药品、食品添加剂或化工产品作为环境激素对环境的污染日益明显。然而，毒理学评价技术尚未建立。尤其是对细胞色素P450等代谢酶激活的代谢产物的毒性评价技术较少。最近有人指出，许多化学品需要重新估算。因此，有必要开发新的技术来评估许多化学品。因此，本项目关注的是开发用于毒理学研究的细胞色素P450过程，而不使用动物如小鼠或大鼠。在实验室（1）、自然界（2）、分子生物学技术（3）和光谱技术（4）中，进行了几项细胞色素P-450对异生物质的酶促分析研究。每个结果都发表在几个期刊上。应该强调的是，在无创评估中已经发展了新技术，使用光谱方法。

It is getting clear that some agricultural medicine, medicine, food additive or chemical product contaminates environment as an environmental hormone. Toxicological assessment technique, however, has not established about them. Especially there is a few technology to estimate toxicity of metabolites, which are activated by metabolic enzyme as cytochrome P450. It is pointed out recently that many chemicals should be estimated again. Therefore it is necessary to develop new technique to assess many chemicals. The present project is thus concerned with the development of cytochrome P450 process for the study of toxicology without using animals as mice or rats. Several experiments have been carried out to study an enzymatic analysis of cytochrome P-450 to xenobiotics in laboratory (1), in natural field (2), expressed by molecular biological technique (3), or using spectroscopic technique (4). Each result has been published in several journal. It should be stressed that new technique has been developed in noinvasive assessment to use spectroscopic method.