Studies on morphogenesis of infection structure and its responses to environment in plant pathogenic fungi

植物病原真菌侵染结构的形态建成及其对环境的响应研究

• 批准号: 09460031
• 负责人: KUBO Yasuyuki
• 金额: $ 4.86万
• 依托单位: Kyoto Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09460031/
• 关键词: Colletotrichum; Infection stucture; Appressoria; Morphogenesis; Signal transduction; Gene expression; Response to environment; Plant pathogenic fungus; イネいもち病菌

1) Isolation and characterization of genes that specifically expressed at appressorium differentiation.In this research, an equalized cDNA library of Colletotrichum leganarium was constructed. By differential screening of this library, 4 genes that preferentially expressed at germination and appressorium formation were isolated. Those genes were characterized by sequencing and expression analysis during appressorium differentiation.Responses to environment and appressorium differentiationIn this research, it became evident that a plant leaf surface substance(s) induces germination of Colletotrichum lagenarium on host plant and this induction was seems to be mediated by cAMP dependent protein kinase signal transduction pathway. A MAP kinase gene was cloned and function of this gene was analyzed by gene disruption experiment. From this, it became evident that a MAP kinase pathway is involved in germination, appressorium formation and invasive growth in host plant. Furthermore, a transcription factor that involved in expression of melanin biosynthesis genes was isolated and characterized. This transcription factor positively regulated two structural melanin biosynthesis genes in a development dependent manner. Also this transcription factor showed very unique structure that contains two different type of DNA binding motifs. This was a first finding of transcription factor among eukaryotic organism.

1)本研究以菜豆炭疽菌（Colletotrichum leganarium）为材料，构建了菜豆炭疽菌（Colletotrichum leganarium）的cDNA文库。通过对文库的差异筛选，分离到4个在种子萌发和附着胞形成过程中优先表达的基因。对环境和附着胞分化的响应本研究发现，植物叶片表面的物质可以诱导西瓜炭疽菌在寄主植物上的萌发，这种诱导作用可能是通过cAMP依赖的蛋白激酶信号转导途径来实现的。克隆了一个MAP激酶基因，并通过基因破坏实验分析了该基因的功能。由此可见，MAP激酶途径参与了寄主植物的萌发、附着胞形成和入侵生长。此外，还分离并鉴定了一个参与黑色素生物合成基因表达的转录因子。该转录因子以发育依赖的方式正向调节两个结构性黑色素生物合成基因。此外，该转录因子显示出非常独特的结构，包含两种不同类型的DNA结合基序。这是在真核生物中首次发现转录因子。

项目成果

Inagaki, A., Takano, Y., Kubo, Y., MIse, K., and Furusawa, I.: "Construction of an equalized cDNA library from Colletotrichum lagenarium and its application to the isolation of differentially expressed genes"Canadian Journal of Microbiology. 46・1. 1-9 (20

Inagaki, A.、Takano, Y.、Kubo, Y.、MIse, K. 和 Furusawa, I.：“Colletotrichum lagenarium 的均衡 cDNA 文库的构建及其在分离差异表达基因中的应用”加拿大杂志微生物学 46・1.

Yoshitaka Takano: "The Colletotrichum lagenarium MAP kinase gene CMK1 regulates diverse aspects for fungal pathogenesis"Molecular Plant-Microbe Interactions. 13. 373-383 (2000)

Yoshitaka Takano：“Colletotrichum lagenarium MAP 激酶基因 CMK1 调节真菌发病机制的多个方面”分子植物-微生物相互作用。

Atsuko Inagaki: "Construction of an equalized cDNA library from Colletotrichum lagenarium and its application to the isolation of differentially expressed genes"Canadian Journal of Microbiology. 46. 150-158 (2000)

Atsuko Inagaki：“Colletotrichum lagenarium 的均衡 cDNA 文库的构建及其在分离差异表达基因中的应用”加拿大微生物学杂志。

Yoshitaka Takano: "The Colletotrichum lagenarium MAP kinase gene CMK1 regulates diverse aspects for fungal pathogenesis"Molecular Plant-Microe Interactions. 13. 374-383 (2000)

Yoshitaka Takano：“Colletotrichum lagenarium MAP 激酶基因 CMK1 调节真菌发病机制的多个方面”分子植物-微生物相互作用。

Takano, Y., Kikuchi, T., Kubo, Y., Hamer, J. E., Mise, K. and Furusawa, I.: "The Colletotrichum lagenarium MAP kinase gene CMK1 regulates diverse aspects for fungal pathogenesis"Molecular Plain-Microbe Interactions. 13・. (2000)

Takano, Y.、Kikuchi, T.、Kubo, Y.、Hamer, J. E.、Mise, K. 和 Furusawa, I.：“Colletotrichum lagenarium MAP 激酶基因 CMK1 调节真菌发病机制的多个方面”分子普通微生物相互作用。 13. (2000)