Basic Research on Molecular Recognition Between Host and Parasite Using Arabidopsis and Filamentous Fungal Pathogen

拟南芥和丝状真菌宿主与寄生虫分子识别的基础研究

• 批准号: 05454060
• 负责人: KUBO Yasuyuki
• 金额: $ 3.71万
• 依托单位: KYOTO PREFECTURAL UNIVERSITY (1994-1995)Kyoto University (1993)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454060/
• 关键词: Arabidopsis; Pathogenicity; Resistance; Pathogen; Molecular biology; Colletotrichum; Alternaria; 感染特異性; 突然変異

To identify genes that determine the host-parasite infection specificity, experimental system using mutants of Arabidopsis and filamentous fungal pathogen that infect Arabidopsis was constructed. To identify plant genes involved in compatibility to the pathogen, Arabidopsis plants derived from mutagen treated M2 seeds were screened for their compatibility to the pathogen alternaria brassicicola. We screened 2,032 plants, however at present, the plants expressing significantly higher resistance or compatibility were not acquired. On the other hand, to identify genes involved in the pathogenicity of the pathogen, we screened for pathogenicity deficient mutants of Colletotrichum higginsianum using REMI (Restriction Enzyme Mediated Integration), a novel technique for making tagging mutation of fungi. Through this technique, we got three mutants. these were 1) the mutant B3-16 expressing weak pathogenicity 2) the mutant E2-9 which can penetrate neither host leaves nor cellulose membranes used for the model substrate for plant cell 3) the mutant S1-27 which can penetrate cellulose membranes, but can not infect host leaves. These mutants are considered to be defective in genes involved in different steps in infectivity. There have been no reports for these type of mutants. Using these mutants, now we can access the isolation of those genes involves in infectivity.

为了鉴定确定宿主 - 寄生虫感染特异性的基因，使用拟南芥的突变体和丝状真菌病原体的实验系统，并构建了感染拟南芥的丝状真菌病原体。为了鉴定涉及与病原体兼容的植物基因，筛选了源自诱变蛋白的M2种子的拟南芥植物，以筛选其与病原体替代植物植物的兼容性。我们筛选了2,032种植物，但是目前，尚未获得表现出明显更高抗性或兼容性的植物。另一方面，为了鉴定与病原体的致病性有关的基因，我们使用remi（限制酶介导的整合）筛选了Colletotrichum higginsianum的致病性缺乏突变体，这是一种新型技术，用于制造真菌的标记突变。通过这项技术，我们得到了三个突变体。这些是1）表达弱致病性的突变体B3-16 2）突变体E2-9不能穿透宿主的叶子，也不能穿透用于植物细胞模型底物的纤维素膜3）可以穿透纤维素膜的突变体S1-27，但不能感染宿主叶。这些突变体被认为是在感染性不同步骤中涉及的基因中有缺陷。没有关于这些类型的突变体的报道。使用这些突变体，现在我们可以访问这些基因的分离涉及感染性。