Studies on the role of the serotonin and NMDA receptors in the plasticity of the developing brain

用NADPH-d组织化学和5-羟色胺免疫组织化学方法比较了一氧化氮合酶(NOS)神经元和5-羟色胺(5-HT)在小鼠脑内的分布。NADPH-黄递酶(NADPH-d)和5-羟色胺(5-HT)阳性胞体和纤维集中分布在嗅前核的内侧部、内侧隔核、Broca斜角带、前脑内侧束、脑室室管膜上丛、膝状体腹外侧核、脚间核的背内侧亚核、中缝大核、中缝隐核、中缝背核、疑核、孤束核和迷走神经背侧运动核。5-羟色胺免疫反应纤维的密度在外侧隔核--泰拉…内广泛减少MU、乳头体内侧核、中缝背核、中缝正中核、中缝隐核、被盖区、小脑和前庭核较多，下丘较少。最明显的是，在胎脑深核和腹侧被盖区之间发现了变性的缠绕纤维。中缝背核5-羟色胺免疫反应强度增加，中缝背核和中缝正中核5-羟色胺免疫反应胞体减少。这些结果提示硫胺素缺乏小鼠5-羟色胺神经元的不同易损性。我们还使用含有一氧化氮合酶神经元的相同标记物NADPH-d组织化学方法检测了硫胺素缺乏小鼠一氧化氮合酶神经元的变化。在硫胺素缺乏小鼠的丘脑、缰核外侧核、中缝背核和前庭内侧核的特定区域，含NADPH-d神经元的胞体数量和纤维密度减少，在乳头体内侧核内侧部分的尾部也发现纤维密度减少。在海马区也检测到含有NADPH-d的神经元的显著丢失。结果提示，在实验性硫胺缺乏时，一氧化氮合酶的影响可能包括脑组织对一氧化氮合酶的某些神经病理易损性。较少

Distributions of nitric oxide synthase (NOS) neurons and serotonin (5-hidroxytyptamine, 5-HT) were compared throughout the mouse brain using NADPH-d histochemistry and 5-HT immunohistochemistry. The areas where NADPH-diaphorase (NADPH-d) and 5-HT positive cell bodies and fibers were much concentrated together included the medial part of the anterior olfactory nucleus, the medial septal nucleus, the diagonal band of Broca, the medial forebrain bundle, the supraependymal plexus of the ventricles, the ventrolateral geniculate nucleus, the dorsomedial subnucleus of the interpeduncular nucleus, the raphe magnus nucleus, the raphe obscurus nucleus, the dorsal raphe nucleus, the ambiguus nucleus, the solitary tract nucleus and the dorsal motor nucleus of the vagus.We examined changes in 5-HT neurons in pyrithiamine-induced thiamine deficiency in mice immunohistochemically. Extensive decreases in the densities of 5-HT-immunoreactive fibers were detected in the lateral septal nucleus, the thala … More mus, the medial mammillary nucleus, the dorsal and the median raphe nuclei, the raphe obscurus nucleus, the tegmental area, the cerebellum and the vestibular nucleus, though only a small decrease was detected in the inferior colliculus. Most remarkably, degenerative winding fibers were detected between the deep mecencephalic nucleus and the ventral tegmental area. Increases in intensity of 5-HT immunoreactivity in the dorsal raphe nucleus and decreases in the number of 5-HT-immunoreactive cell bodies in the dorsal and the median raphe nuclei were detected. These results suggest the differential vulnerability of 5-HT neurons in thiamine-deficient mice.Changes in NOS neurons in thiamine-deficient mice were also examined histochemically using NADPH-d, an identical marker for NOS containing neurons. Decreases in the number of cell bodies and the densities of fibers in NADPH-d- containing neurons were detected in particular areas of the thalamus, the lateral habenular nucleus, the dorsal raphe nucleus and the medial vestibular nucleus, and decreases in the density of the fibers was detected also in the caudal part of the medial portion of the medial mammillary nucleus in thiamine-deficient mice. A marked loss of NADPH-d-containing neurons was also detected in the hippocampus. The results suggest the possibility that the influence of NOS includes some neuropathological vulnerability of the brain to NOS in experimental thiamine deficiency. Less