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Studies on the role of the serotonin and NMDA receptors in the plasticity of the developing brain

血清素和 NMDA 受体在大脑发育可塑性中作用的研究

基本信息

批准号：
09470184
负责人：
TAKEUCHI Yoshihiro
金额：
$ 2.75万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470184/
关键词：
Serotonin NMDA receptor Nitric oxide thiamine-deficiency Plasticity NADPH-α

项目摘要

Distributions of nitric oxide synthase (NOS) neurons and serotonin (5-hidroxytyptamine, 5-HT) were compared throughout the mouse brain using NADPH-d histochemistry and 5-HT immunohistochemistry. The areas where NADPH-diaphorase (NADPH-d) and 5-HT positive cell bodies and fibers were much concentrated together included the medial part of the anterior olfactory nucleus, the medial septal nucleus, the diagonal band of Broca, the medial forebrain bundle, the supraependymal plexus of the ventricles, the ventrolateral geniculate nucleus, the dorsomedial subnucleus of the interpeduncular nucleus, the raphe magnus nucleus, the raphe obscurus nucleus, the dorsal raphe nucleus, the ambiguus nucleus, the solitary tract nucleus and the dorsal motor nucleus of the vagus.We examined changes in 5-HT neurons in pyrithiamine-induced thiamine deficiency in mice immunohistochemically. Extensive decreases in the densities of 5-HT-immunoreactive fibers were detected in the lateral septal nucleus, the thala … More mus, the medial mammillary nucleus, the dorsal and the median raphe nuclei, the raphe obscurus nucleus, the tegmental area, the cerebellum and the vestibular nucleus, though only a small decrease was detected in the inferior colliculus. Most remarkably, degenerative winding fibers were detected between the deep mecencephalic nucleus and the ventral tegmental area. Increases in intensity of 5-HT immunoreactivity in the dorsal raphe nucleus and decreases in the number of 5-HT-immunoreactive cell bodies in the dorsal and the median raphe nuclei were detected. These results suggest the differential vulnerability of 5-HT neurons in thiamine-deficient mice.Changes in NOS neurons in thiamine-deficient mice were also examined histochemically using NADPH-d, an identical marker for NOS containing neurons. Decreases in the number of cell bodies and the densities of fibers in NADPH-d- containing neurons were detected in particular areas of the thalamus, the lateral habenular nucleus, the dorsal raphe nucleus and the medial vestibular nucleus, and decreases in the density of the fibers was detected also in the caudal part of the medial portion of the medial mammillary nucleus in thiamine-deficient mice. A marked loss of NADPH-d-containing neurons was also detected in the hippocampus. The results suggest the possibility that the influence of NOS includes some neuropathological vulnerability of the brain to NOS in experimental thiamine deficiency. Less

用NADPH-d组织化学和5-HT免疫组织化学方法比较了小鼠脑内一氧化氮合酶（NOS）神经元和5-羟色胺（5-HT）的分布。NADPH-d和5-HT阳性胞体和纤维集中的区域包括嗅前核内侧部、隔内侧核、Broca斜角带、前脑内侧束、脑室室管膜上丛、膝状体腹外侧核、脚间核背内侧亚核、中缝大核、用免疫组织化学方法观察了吡啶硫胺致小鼠硫胺素缺乏时5-HT神经元的变化。5-HT免疫反应阳性纤维密度在外侧隔核、丘脑、小脑和小脑中广泛减少。 ...更多信息 mus、乳头体内侧核、中缝背核和中缝核、中缝隐核、被盖区、小脑和前庭核，但下丘仅检测到少量减少。最值得注意的是，在中脑深核和腹侧被盖区之间检测到退行性缠绕纤维。中缝背核5-HT免疫反应强度增加，中缝背核和正中核5-HT免疫反应细胞体的数量减少。这些结果表明，硫胺素缺乏小鼠的5-HT神经元的差异易损性。NOS神经元的变化，硫胺素缺乏小鼠也进行了组织化学检测用NADPH-d，一个相同的标记含NOS神经元。在特定区域的丘脑，外侧缰核，中缝背核和前庭内侧核的细胞体的数量和NADPH-d-含神经元的纤维密度的减少，并检测到减少的纤维密度也在尾部的内侧乳头核的内侧部分在硫胺素缺乏小鼠。在海马中也检测到含NADPH-d的神经元的显著损失。结果表明，NOS的影响可能包括一些神经病理脆弱性的大脑NOS在实验性硫胺素缺乏症。少