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HISTOCHEMICAL STUDIES ON TRANSMITTER-RELATED NEURON SYSTEM UNDER EXPERIMENTALLY INDUCED SEIZURES

实验诱发癫痫发作下递质相关神经元系统的组织化学研究

基本信息

批准号：
63570452
负责人：
TAKEUCHI Yoshihiro
金额：
$ 1.28万
依托单位：
KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570452/
关键词：
Seizure Serotonin Mouse Neostriatum Immunohistochemistry 痙攣

项目摘要

Serotonin neurons in the brainstem give rise to highly branched fibers which innervate virtually the entire central nervous system, comprising the most expatisive neuronal network among other neurotransmitters. The aim of this project is to examine changes occurring in the serotonin neuron system in experimentally induced convulsions by means of a modified immunoperoxidase method. To obtain quantitative data on the density or serotonergic innervation, the total length of serotonin-immunoreactive fibers was calculated using the MOP system. A marked increase in serotonin immunoreactivity was observed at the rostral and intermediate levels or the neostriatum of mice kept at high temperature (50ﾟC) with no convulsions, and a significant reduction in serotonin immunoreactivity was verified throughout the neostriatum of mice which had hyperthermia-induced seizures. in the case or pilocarpine-induced convulsions, a significant reduction in serotonin immunoreactivity was observed at the rostral and caudal levels of the neostriatum or mice which had generalized seizures due to the systemic administration of pilocarpine (350 mg/kg). In both experimental convulsion models, the serotonin immunoreactivity remained unchanged in the paleostriatum and neocortex and the degree of serotonin reduction in the neostriatum was significantly greater in hyperthermia-induced convulsions than that in pilocarpine-induced convulsions. These results suggest that striatal serotonin may be an important mediater in the mechanism of convulsions and that more precise regional analysis using quantitative histochemical methods is essential for elucidation of the mechanism underlying human neurological disorders.

脑干中的5-羟色胺神经元产生高度分支的纤维，其几乎支配整个中枢神经系统，包括其他神经递质中最详尽的神经元网络。本项目的目的是通过改良的免疫过氧化物酶法检测实验诱导的惊厥中5-羟色胺神经元系统发生的变化。为了获得有关密度或肾上腺素能神经支配的定量数据，使用MOP系统计算肾上腺素免疫反应性纤维的总长度。在保持在高温（50 ℃）下而没有惊厥的小鼠的嘴侧和中间水平或新纹状体观察到5-羟色胺免疫反应性显著增加，并且在具有高血压诱导的癫痫发作的小鼠的整个新纹状体中证实了5-羟色胺免疫反应性的显著降低。在毛果芸香碱诱导的惊厥的情况下，在新纹状体的嘴侧和尾侧水平或由于全身施用毛果芸香碱（350 mg/kg）而具有全身性癫痫发作的小鼠中观察到5-羟色胺免疫反应性的显著降低。在这两个实验性惊厥模型中，5-羟色胺免疫反应性保持不变，在古纹状体和新皮质和5-羟色胺减少的程度在新纹状体是显着更大的高血压引起的惊厥比在匹罗卡品引起的惊厥。这些结果表明，纹状体5-羟色胺可能是一个重要的中介机构的机制，惊厥和更精确的区域分析，使用定量组织化学方法是必不可少的阐明人类神经系统疾病的机制。