Development of Perfluorochemicals in Cardiovascular Surgery

全氟化合物在心血管外科中的发展

• 批准号: 09470278
• 负责人: MATSUURA Yuichiro
• 金额: $ 4.74万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470278/
• 关键词: cardiac ischemic; reperfusion injury; Perfluorochemicals; cardiopulmonary bypass; cardiac physiological analysis

This study was designed to assess the effect of Perfluorotributylamine/Pluronic F-68 Stem-Emulsion (FC43se) administration on post-ischemic cardiac function after CPB (cardiopulmonary bypass).(Methods) After 60min CPB, the beagle dog heart was submitted to warm ischemia state for 30min at 36ﾟC.Then the heart was made to resume heart beating. Experimental groups were divided into two groups. In control(C) group (n=19) and FC43se-administration (FC) group (n=14), 10m1 of saline and FC43se were infused into aortic root just before aortic unclamping, respectively. The physiological parameters of left ventncular(LV) function (Emax, maxdp/dt ) were measured at 3 time points (before CPB, 30min after re-beating, 60min after re-beating). Left ventricular specimens were taken for histologic examination at 30 min and 60min after re-beating.(Results) 1. ESPVR=Emax : FC group (before CPB 19.0*1.7mmHg/ml : 30min after re-beating 14.2*1.4 ; p<.0001 vs C Group, recovery rate=RR 73.4*11.3% : p<.000l vs C Group : 60min after re-beating 18.2*2.7, RR 96.0*7.1%). C group (before CPB 20.5*3.4 : 30min after re-beating 10.7*2.0, RR 52.9*11.4% : 60min after re-beating 18.2*3.9, RR 88.9*8.1%). 2.max dpfdt : FC group (before CPB 3555.3*349.3mmHg/s : 30mm after re-beating 2119.4*536.2, RR 59.9*14.5% 60min after re-beating 2859.7*126.4, RR 81.2*15.9%). C group (before CPB 2834.1*427.6 : 30min after re-beating 1357.5*107.6, RR 49.8*20.3% : 60min after re-beating 2436.0*142.7, RR 85.9*16.9%). No significant differences in max dp/dt values between two groups were detected. And no histological change in myocardiac tissues were detected in all specimens.(Conclusion) Although FC43se administration had effect on early recovery of the cardiac function after CPB, the satisfactory effect of FC43se could not be proved.

本研究旨在评估全氟三丁基胺/Pluronic F-68茎乳剂（FC43se）给药对体外循环（CPB）术后缺血心功能的影响。（方法）在CPB 60min后，将beagle犬心脏置于36oc热缺血状态30min。然后心脏又恢复了跳动。实验组分为两组。对照组(C)组（n=19）和FC43se给药组（n=14）在主动脉解夹前分别向主动脉根部注入10m1生理盐水和FC43se。在CPB前、复跳后30min、复跳后60min 3个时间点测量左室功能生理参数Emax、maxdp/dt。于复跳后30min和60min分别取左心室标本进行组织学检查。(结果)1。ESPVR=Emax: FC组（CPB前19.0*1.7mmHg/ml：复跳后30min 14.2*1.4; p< 0.0001，恢复率=RR 73.4*11.3%; p< 0.000l，与C组：复跳后60min 18.2*2.7, RR 96.0*7.1%）。C组（CPB前20.5*3.4；复跳后30min 10.7*2.0, RR 52.9*11.4%；复跳后60min 18.2*3.9, RR 88.9*8.1%）。2.最大dpfdt: FC组（CPB前3555.3*349.3mmHg/s，复跳30mm后2119.4*536.2,RR 59.9*14.5%，复跳60min后2859.7*126.4,RR 81.2*15.9%）。C组（CPB前2834.1*427.6；复跳后30min 1357.5*107.6, RR 49.8*20.3%；复跳后60min 2436.0*142.7, RR 85.9*16.9%）。两组间最大dp/dt值无显著差异。各组心肌组织均未见组织学改变。（结论）虽然给药FC43se对CPB术后心功能的早期恢复有一定作用，但其效果尚不能令人满意。