The physiological adaptation for chronic nonpulsatile perfusion - We will clarify the mechanism of the physiological adaptation molecular biologically -

慢性非脉动灌注的生理适应 - 我们将从分子生物学角度阐明生理适应的机制 -

• 批准号: 09470280
• 负责人: TOMINAGA Ryuji
• 金额: $ 8.26万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470280/
• 关键词: Pulsatile; nonpulsatile; EDNO; L-NAME; peripheral vasculature resistance; hyperemia; 急性実験; 慢性実験; 人工循環; 血管内皮細胞; 拍動性; Nitric Oxide; 一酸化窒素; 分子生物学

Before we start the experiment of the animal model of chronic pulsatile and nonpulsatile blood flow, we investigated the effects of pulsatility in blood flow on endothelium-derived nitric oxide (EDNO) release in the peripheral vasculature with the experiment of the acute animal model.The basal and flow stimulated EDNO release were compared between pulsatile and nonpulsatile systemic flows before and after the administratrion of NO synthase inhibitor NィイD1GィエD1-monomethyl-L-arginine (L-NAME) (1) Peripheral vascular resistance (PVR) was significantly lower in pulsatile flow than in nonpulsatile flow, but this difference disappeared after L-NAME. The percent increase in PVR by L-NAME was significantly larger in pulsatile flow. (2) In reactive hyperemiain the hindlimb, the peak flow did not differ ; however, both the repayment flow and the duration were significantly larger pulsatile flow These data indicated that pulsatility significantly enhances the basal and flow-stimulated EDNO release in the periperal vasculature under in vivo conditions.Before we start the experiment of the animal model of chronic pulsatile and nonpulsatile blood flow, we investigated the effects of pulsatility in blood flow on EDNO release in the peripheral vasculature with the experiment of the acute animal model.

在开始我们的慢性搏动性和非脉动血液流动的动物模型之前，我们研究了血流中脉动性对外周脉管中内皮衍生的一氧化氮（EDNO）释放的脉动性与急性动物模型之间的实验和流动型的脉动释放的实验。抑制剂NYI D1GIE D1-含量甲基-L-精氨酸（L-NAME）（1）脉冲流动的外周血管抗性（PVR）明显低于非脉冲流动，但是L-NAME后这种差异消失了。在脉冲流中，L-NAME的PVR百分比明显更大。 （2）在后肢反应性充血，峰流量没有差异；然而，这些数据表明，在体内条件下，脉动性的脉动流量和持续时间都明显更大，这些数据表明，脉冲性显着增强了外周血管中的基本和流动刺激的EDNO释放。在我们开始在持续脉动血流的动物模型的实验之前，我们对脉动血流的动物模型进行了研究，我们对脉冲的效果进行了研究，我们研究了血液的效果。急性动物模型的实验。

项目成果

Ryuji Tominaga: "Oxygen transport and utilization in chronic nonpulsatile"J Artif Organs. 2. 24-29 (1999)

Ryuji Tominaga：“慢性非搏动性的氧气输送和利用”J Artif Organs。

Toshihide Nakano et al.: "Pulsatile flow enhances endothelium-derived nitric oxide release in the peripheral vasculature"Am J. Physiol. (in press).

Toshihide Nakano 等人：“脉动流增强外周脉管系统中内皮源性一氧化氮的释放”Am J. Physiol。

Toshihide Nakano et al.: "Pulsatile flow enhances endothelium-derived nitric oxide release in the peripheral vasonlature"Am J.Physiol. (in press).

Toshihide Nakano 等人：“脉动流增强外周血管中内皮源性一氧化氮的释放”Am J.Physiol。

Ryuji Tominaga: "Oxygen transport and utilization in chronic nonpulsatile blood flow"J Artif Organs. 2. 24-29 (1999)

Ryuji Tominaga：“慢性非脉动血流中的氧气输送和利用”J Artif Organs。