Preparation of phosphated and sulfated polysaccharides and polyphenols and their physiological activities

点击翻译按钮获取中文摘要

Natural and chemically synthesized polysaccharides were sulfated to investigate its biological activities. First, heparanase inhibition activities were investigated, It was found that (l(]SY.tri-substituted left.[)5)-beta-D-glucofuranan sulfate, (l(]SY.tri-substituted left.[)5)-beta-D-galactofuranan sulfate, xylan sulfate, and curdlan sulfate have heparanase inhibition activities. Then, anticoagulant activities were measured by an APTT (activated partial thromboplastin time). Among the tested compounds, (1(]SY.tri-substituted left.[)5)-beta-D-glucofuranan sulfate, (1(]SY.tri-substituted left.[)5)-beta-D-galactofuranan sulfate, xylan sulfate, and curdlan sulfate have low anticoagulant activities. Furthermore, inhibition activities of B 16BL6 lung metastasis by the four polysaccharides, i. e., (1(]SY.tri-substituted left.[)5)- beta-D-glucofuranan sulfate, (1(]SY.tri-substituted left.[)5)-beta-D-galactofuranan sulfate, xylan sulfate, and curdlan sulfate is in the order : (1(]SY.tri-substituted left.[)5)-beta-D-galactofuranan sulfate> (l(]SY.tri-substituted left.[)5)-beta-D-Glucofuranan sulfate> xylan sulfate, curdlan sulfate. It was found that (l(]SY.tri-substituted left.[)5)-beta-D-glucofuranan sulfate, (1(]SY.tri-substituted left.[)5)-beta-D-galactofuranan sulfate, and xylan sulfate have not only low anticoagulant activities but also high inhibition actibities of Bi 6BL6 lung metastasis.